Mirfasihi Armin, Malek Afzali Beheshteh, Ebrahimi Zadeh Hosna, Sanjari Khashayar, Mir Maziar
Assistant Professor, Periodontology Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Associate Professor, Department of Pediatric Dentistry, Dental Research Center, Research Institute of Dental Science, Department of Pediatric Dentistry, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2020 Fall;11(4):405-410. doi: 10.34172/jlms.2020.64. Epub 2020 Oct 3.
This study aimed to assess the effect of photodynamic therapy (PDT) and chitosan separately and in combination on . This in vitro experimental study evaluated 216 microbial samples in 6 groups. First, 5 µL of 0.5 McFarland standard suspension of S. mutans was added to each well of an ELISA microplate; 100 µL of Mueller Hinton broth was also added to each well; 180 wells contained suspension while 36 wells were devoid of bacteria. Group 1 served as the negative control and had no bacteria. Group 2 served as the positive control and in the positive control wells did not undergo any intervention. In groups 3 and 4, PDT with a 50 mW low-level laser was performed for 30 and 40 seconds respectively. In group 5, 3 mg/mL of chitosan (100 µL) was used. In group 6, 3 mg/mL (100 µL) of chitosan was used in combination with PDT (50 mW laser for 30 seconds). The laser was irradiated under aseptic conditions at a 660 nm wavelength with 50 mW power. Data were analyzed using one-way ANOVA and Tukey's test. PDT combined with chitosan showed maximum bactericidal effect followed by PDT for 40 seconds and chitosan groups ( < 0.05). PDT for 30 seconds showed a minimum bactericidal effect ( < 0.05). All pairwise comparisons revealed significant differences ( < 0.001). Chitosan and PDT alone can be used to decrease the count. However, their combined use has a greater bactericidal effect on .
本研究旨在分别评估光动力疗法（PDT）和壳聚糖单独及联合应用对……的影响。这项体外实验研究评估了6组中的216个微生物样本。首先，将5µL变形链球菌0.5麦氏标准悬液添加到酶联免疫吸附测定（ELISA）微孔板的每个孔中；同时向每个孔中添加100µL米勒-欣顿肉汤；180个孔含有悬液，而36个孔无细菌。第1组作为阴性对照，无细菌。第2组作为阳性对照，阳性对照孔未进行任何干预。在第3组和第4组中，分别用50mW低强度激光进行30秒和40秒的光动力疗法。在第5组中，使用3mg/mL的壳聚糖（100µL）。在第6组中，将3mg/mL（100µL）的壳聚糖与光动力疗法（50mW激光照射30秒）联合使用。激光在无菌条件下以660nm波长、50mW功率进行照射。数据采用单因素方差分析和Tukey检验进行分析。光动力疗法联合壳聚糖显示出最大杀菌效果，其次是40秒的光动力疗法组和壳聚糖组（P<0.05）。30秒的光动力疗法显示出最小杀菌效果（P<0.05）。所有两两比较均显示出显著差异（P<0.001）。壳聚糖和单独的光动力疗法均可用于减少……计数。然而，它们联合使用对……具有更大的杀菌效果。
