Yamanaka Mikihiro, Shirakawa Jun-Ichi, Ohno Rei-Ichi, Shinagawa Masatoshi, Hatano Kota, Sugawa Hikari, Arakawa Shoutaro, Furusawa Chisato, Nagai Mime, Nagai Ryoji
Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto 869-1404, Japan; Healthcare Business Development Department I, Medical and Healthcare Business Development Unit, Business Solution Company, SHARP Corporation, 2613-1 Ichinomoto-cho, Tenri, Nara 632-8567, Japan.
Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso-gun, Kumamoto 869-1404, Japan.
J Clin Biochem Nutr. 2016 Mar;58(2):130-4. doi: 10.3164/jcbn.15-131. Epub 2016 Jan 22.
Although soft-shelled turtle eggs (STE) have been used as a folk medicine for revitalization and the prevention of lifestyle-related diseases, the scientific evidence to support the use of STE in this manner is scarce. To clarify the physiological evidence, STE was administered to diabetic rats and the inhibitory effects on the formation of advanced glycation end-products (AGEs), which are known to increase with the progression of lifestyle-related diseases, were examined. STE and citric acid were administered to diabetic rats for 3 months, and serum N (ε)-(carboxymethyl)lysine (CML) contents were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Although the administration of STE did not affect the body weight, glycoalbumin or ketone body levels, it significantly reduced the serum level of CML. The accumulation of AGEs, which was measured by fluorescence intensity in the auricle skin and the lower gums, was also reduced by the administration of STE to a similar extent to that observed with citric acid. This report provides the first evidence that the oral administration of STE reduces the formation of AGEs, suggesting that one of the health effects of STE may be the inhibition of AGEs formation.
尽管甲鱼蛋一直被用作一种用于强身健体和预防生活方式相关疾病的民间药物，但以这种方式使用甲鱼蛋的科学证据却很少。为了阐明其生理证据，将甲鱼蛋给予糖尿病大鼠，并检测其对晚期糖基化终产物（AGEs）形成的抑制作用，已知AGEs会随着生活方式相关疾病的进展而增加。将甲鱼蛋和柠檬酸给予糖尿病大鼠3个月，通过液相色谱-串联质谱法（LC-MS/MS）测量血清N-（ε）-（羧甲基）赖氨酸（CML）含量。尽管给予甲鱼蛋对体重、糖化白蛋白或酮体水平没有影响，但它显著降低了血清CML水平。通过耳廓皮肤和下牙龈的荧光强度测量的AGEs积累，在给予甲鱼蛋后也降低到了与柠檬酸相似的程度。本报告首次提供了口服甲鱼蛋可减少AGEs形成的证据，表明甲鱼蛋的健康益处之一可能是抑制AGEs的形成。
