Azmath Pasha, Baker Syed, Rakshith Devaraju, Satish Sreedharamurthy
Bionano Technological Laboratory, Department of Studies in Microbiology, University of Mysore, Manasagangotri, India.
Saudi Pharm J. 2016 Mar;24(2):140-6. doi: 10.1016/j.jsps.2015.01.008. Epub 2015 Jan 21.
Mycosynthesis of silver nanoparticles was achieved by endophytic Colletotrichum sp. ALF2-6 inhabiting Andrographis paniculata. Well dispersed nanoparticles were characterized using UV-Visible spectrometry with maximum absorption conferring at 420 nm. FTIR analysis revealed possible biomolecules reducing the metal salt and stabilization of nanoparticles. XRD analysis depicted the diffraction intensities exhibiting between 20 and 80 °C at 2theta angle thus conferring the crystalline nature of nanoparticles. Morphological characteristic using TEM revealed the polydispersity of nanoparticles with size ranging from 20 to 50 nm. Synthesized nanoparticles exhibited bactericidal activity against selected human pathogens. Nanoparticles mode of action was carried out to reveal DNA damage activity. Thus the present investigation reports facile fabrication of silver nanoparticles from endophytic fungi.
通过栖息于穿心莲中的内生炭疽菌属（Colletotrichum sp.）ALF2-6实现了银纳米颗粒的真菌合成。使用紫外可见光谱对分散良好的纳米颗粒进行表征，其最大吸收峰位于420nm处。傅里叶变换红外光谱（FTIR）分析揭示了可能参与还原金属盐和稳定纳米颗粒的生物分子。X射线衍射（XRD）分析表明，在2θ角为20至80°C之间呈现出衍射强度，从而证实了纳米颗粒的晶体性质。使用透射电子显微镜（TEM）进行的形态学特征分析显示，纳米颗粒具有多分散性，尺寸范围为20至50nm。合成的纳米颗粒对选定的人类病原体表现出杀菌活性。对纳米颗粒的作用模式进行了研究以揭示其DNA损伤活性。因此，本研究报告了从内生真菌中简便制备银纳米颗粒的方法。
