Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, South Korea.
Department of Food Science and Engineering, College of Agricultural, Yanbian University, Yanji, Jilin, People's Republic of China.
Int J Nanomedicine. 2019 May 9;14:3427-3438. doi: 10.2147/IJN.S200817. eCollection 2019.
Biogenic silver nanoparticles (AgNPs) have wider range of biomedical applications. The present work synthesized Tp-AgNPs using mycelial extract of endophytic fungus (MEEF), characterized, and analyzed for antibacterial, anti-proliferation and cell wounding healing activities. The synthesized Tp-AgNPs were characterized by UV-visible spectrophotometer (UV-Vis), field emission transmission electron microscopy (FETEM) with energy-dispersive X-ray spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), particle size analysis (PSA) and X-ray diffraction (XRD). Further, antibacterial activity was determined by Kirby-Bauer test and anti-proliferation activity was tested in human lung carcinoma A549 by water-soluble tetrazolium and flow cytometer assay. In addition, cell wounding healing activity was determined by scratch assay. UV-Vis results displayed a strong absorption peak from 390 nm to 420 nm, which indicated the successful synthesis of Tp-AgNPs. FETEM-EDS results indicated the round and triangle shaped Tp-AgNPs with the average size of 25 nm in accordance with PSA. FTIR analysis indicated the involvement of various functional molecules from MEEF in the synthesis of Tp-AgNPs. XRD result proved nature of Tp-AgNPs as a high-quality crystal. The Tp-AgNPs significantly inhibited the growth of bacterial pathogens at the minimal inhibitory concentration of 16.12 μg.mL for Gram, and 13.98 μg.mL for Gram bacteria. Further, Tp-AgNPs (2 μg.mL) showed a strong anti-proliferation effect in A549. Interestingly, Tp-AgNPs was not cytotoxic to normal NIH3T3 cells. In addition, the NPs exhibited a strong cell wounding healing activity. This work biosynthesized AgNPs with strong antibacterial, anticancer and cell wound healing properties using endophytic fungus .
生物成因银纳米粒子（AgNPs）在更广泛的生物医学应用中具有优势。本工作使用内生真菌（MEEF）的菌丝体提取物合成 Tp-AgNPs，对其进行了表征，并分析了其抗菌、抗增殖和细胞创伤愈合活性。通过紫外可见分光光度计（UV-Vis）、场发射透射电子显微镜（FETEM）结合能谱（EDS）、傅里叶变换红外光谱（FTIR）、粒径分析（PSA）和 X 射线衍射（XRD）对合成的 Tp-AgNPs 进行了表征。此外，通过 Kirby-Bauer 试验测定了抗菌活性，通过水溶性四唑盐和流式细胞术测定了人肺癌 A549 细胞的抗增殖活性。另外，通过划痕试验测定了细胞创伤愈合活性。UV-Vis 结果显示，从 390nm 到 420nm 有一个很强的吸收峰，这表明成功合成了 Tp-AgNPs。FETEM-EDS 结果表明，Tp-AgNPs 呈圆形和三角形，平均粒径为 25nm，与 PSA 结果一致。FTIR 分析表明，MEEF 中的各种功能分子参与了 Tp-AgNPs 的合成。XRD 结果证明 Tp-AgNPs 是一种高质量的晶体。Tp-AgNPs 在最低抑菌浓度（16.12μg·mL-1 对革兰氏阳性菌，13.98μg·mL-1 对革兰氏阴性菌）时显著抑制了病原菌的生长。此外，Tp-AgNPs（2μg·mL-1）在 A549 中表现出很强的抗增殖作用。有趣的是，Tp-AgNPs 对正常 NIH3T3 细胞没有细胞毒性。此外，该纳米粒子还表现出很强的细胞创伤愈合活性。本工作利用内生真菌生物合成了具有强抗菌、抗癌和细胞创伤愈合特性的 AgNPs。
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