Department of P. G. Studies and Research in Microbiology, School of Biosciences, Kuvempu University, Jnanasahyadri, Shivamogga Dist, Shankaraghatta, 577451, Karnataka, India.
Department of P. G. Studies and Research in Biochemistry, Kuvempu University, Jnanasahyadri, Shivamogga Dist, Shankaraghatta, 577451, Karnataka, India.
Arch Microbiol. 2023 May 2;205(5):217. doi: 10.1007/s00203-023-03549-1.
The primary objective of the current investigation was the biosynthesis of Phy-AgNPs by the endophytic fungus Phyllosticta owaniana (extracted from Abrus precatorius) and the evaluation of the secondary metabolites from the ethyl acetate extract of P. owaniana cultivated by submerged fermentation. Utilizing bioanalytical strategies, Phy-AgNPs were characterized. The UV-visible spectrophotometer analysis revealed an absorption spectrum with a peak at 420 nm, thus validating the Phy-AgNPs synthesis. The FTIR analysis revealed peaks correlating to various potential functional groups, suggesting that Phy-AgNPs have been reduced and capped. SEM-EDAX and HR-TEM analyses demonstrated the spherical shape of Phy-AgNPs, and the 3 keV EDAX analysis confirmed the existence of silver atoms. XRD analyses showed the Phy-AgNPs crystalline structure. The size and the stability of synthesized Phy-AgNPs (65.81 nm) were measured by DLS and Zeta potential studies. While the ethyl acetate extract was analyzed with GC-MS and FTIR for secondary metabolites. The synthesized Phy-AgNPs showed effective antibacterial activity against Pseudomonas aeruginosa (15.1 ± 0.17 mm, 10 mg/mL), while the antifungal activity of Phy-AgNPs inhibited the growth of Candida albicans extremely efficiently (12.16 ± 0.28 mm, 10 mg/mL). Phy-AgNPs were evaluated for a variety of biomedical properties in which they showed significant activity. In a cell viability assay using the MTT assay, Phy-AgNPs exhibited a cytotoxic impact of up to 30.67% and 34.53% when 200 µg/mL were detected. In both in vitro and in vivo anti-inflammatory examinations, nanoparticles (NPs) exhibited a significant anti-inflammatory effect. These findings support the pharmaceutical and biomedical properties of the synthesized Phy-AgNPs.
本研究的主要目的是通过内生真菌 Phyllosticta owaniana（从鸡骨草 Abrus precatorius 中提取）生物合成 Phy-AgNPs，并评估通过浸液发酵培养的 P. owaniana 乙酸乙酯提取物中的次级代谢产物。利用生物分析策略对 Phy-AgNPs 进行了表征。紫外可见分光光度计分析显示，在 420nm 处有一个吸收峰，从而验证了 Phy-AgNPs 的合成。傅里叶变换红外（FTIR）分析显示出与各种潜在功能基团相关的峰，表明 Phy-AgNPs 已被还原并被封端。SEM-EDAX 和高分辨率透射电子显微镜（HR-TEM）分析表明 Phy-AgNPs 呈球形，3keV 的 EDAX 分析证实存在银原子。X 射线衍射（XRD）分析表明 Phy-AgNPs 具有晶体结构。通过 DLS 和 Zeta 电位研究测量了合成的 Phy-AgNPs 的尺寸和稳定性（65.81nm）。同时，利用 GC-MS 和 FTIR 对次级代谢产物进行了分析。合成的 Phy-AgNPs 对铜绿假单胞菌（15.1±0.17mm，10mg/mL）表现出有效的抗菌活性，而 Phy-AgNPs 的抗真菌活性则能非常有效地抑制白色念珠菌的生长（12.16±0.28mm，10mg/mL）。Phy-AgNPs 的各种生物医学特性进行了评估，结果表明它们具有显著的活性。在使用 MTT 法的细胞活力测定中，当检测到 200μg/mL 的 Phy-AgNPs 时，Phy-AgNPs 表现出高达 30.67%和 34.53%的细胞毒性。在体内和体外抗炎研究中，纳米粒子（NPs）表现出显著的抗炎作用。这些发现支持合成的 Phy-AgNPs 的药用和生物医学特性。
