Hrv Rajkumar, Devaki Ramakrishna, Kandi Venkataramana
Department of Microbiology, Kamineni Academy of Medical Sciences & Research Centre.
Department of Biochemistry, Kamineni Academy of Medical Sciences & Research Centre.
Cureus. 2016 Feb 11;8(2):e489. doi: 10.7759/cureus.489.
Among the various pathogenic determinants shown by microorganisms hemagglutination and hemolysin production assume greater significance in terms of laboratory identification. This study evaluated the hemagglutination and hemolytic activity of various bacterial isolates against different blood groups. One hundred and fifty bacterial strains, isolated from clinical specimens like urine, pus, blood, and other body fluids were tested for their hemagglutinating and hemolytic activity against human A, B, AB, and O group red blood cells. Among the 150 isolates 81 were Escherichia coli, 18 were Klebsiella pneumoniae, 19 were Pseudomonas aeruginosa, 10 were Pseudomonas spp, six were Proteus mirabilis, and the rest 16 were Staphylococcus aureus. Nearly 85% of the isolates agglutinated A group cells followed by B and AB group (59.3% and 60.6% respectively). Least number of isolates agglutinated O group cells (38.0%). When the hemolytic activity was tested, out of these 150 isolates 79 (52.6%) hemolyzed A group cells, 61 (40.6%) hemolyzed AB group cells, 46 (30.6%) hemolyzed B group cells, and 57 (38.6%) isolates hemolyzed O group cells. Forty-six percent of the isolates exhibited both hemagglutinating and hemolytic property against A group cells, followed by B and AB group cells (28.6% and 21.3% respectively). Least number of isolates i.e., 32 (21.3%) showed both the properties against O group cells. The isolates showed wide variation in their hemagglutination and hemolytic properties against different combinations of human blood group cells. The study highlights the importance of selection of the type of cells especially when human RBCs are used for studying the hemagglutination and hemolytic activity of bacterial isolates because these two properties are considered as characteristic of pathogenic strains.
在微生物所表现出的各种致病决定因素中，血凝和溶血素产生在实验室鉴定方面具有更重要的意义。本研究评估了各种细菌分离株对不同血型的血凝和溶血活性。对从尿液、脓液、血液和其他体液等临床标本中分离出的150株细菌菌株，检测它们对人A、B、AB和O型红细胞的血凝和溶血活性。在这150株分离株中，81株为大肠杆菌，18株为肺炎克雷伯菌，19株为铜绿假单胞菌，10株为假单胞菌属，6株为奇异变形杆菌，其余16株为金黄色葡萄球菌。近85%的分离株凝集A型细胞，其次是B型和AB型（分别为59.3%和60.6%）。凝集O型细胞的分离株数量最少（38.0%）。当检测溶血活性时，在这150株分离株中，79株（52.6%）使A型细胞溶血，61株（40.6%）使AB型细胞溶血，46株（30.6%）使B型细胞溶血，57株（38.6%）分离株使O型细胞溶血。46%的分离株对A型细胞同时表现出血凝和溶血特性，其次是B型和AB型细胞（分别为28.6%和21.3%）。最少数量的分离株即32株（21.3%）对O型细胞表现出这两种特性。这些分离株对人血型细胞的不同组合在血凝和溶血特性方面表现出很大差异。该研究强调了选择细胞类型的重要性，尤其是在使用人红细胞研究细菌分离株的血凝和溶血活性时，因为这两种特性被视为致病菌株的特征。
