Ranjbar Sistani Nima, Kaul Hans-Peter, Desalegn Getinet, Wienkoop Stefanie
Molecular Systems Biology, Department of Ecogenomics and Systems Biology, Faculty of Life Sciences, University of Vienna, Vienna, Austria.
Department of Crop Sciences, University of Natural Resources and Life Sciences, ViennaVienna, Austria.
Front Plant Sci. 2017 Nov 15;8:1961. doi: 10.3389/fpls.2017.01961. eCollection 2017.
In field peas, ascochyta blight is one of the most common fungal diseases caused by Despite the high diversity of pea cultivars, only little resistance has been developed until to date, still leading to significant losses in grain yield. Rhizobia as plant growth promoting endosymbionts are the main partners for establishment of symbiosis with pea plants. The key role of as an effective nitrogen source for legumes seed quality and quantity improvement is in line with sustainable agriculture and food security programs. Besides these growth promoting effects, symbiosis has been shown to have a priming impact on the plants immune system that enhances resistance against environmental perturbations. This is the first integrative study that investigates the effect of bv. ) on phenotypic seed quality, quantity and fungal disease in pot grown pea () cultivars with two different resistance levels against through metabolomics and proteomics analyses. In addition, the pathogen effects on seed quantity components and quality are assessed at morphological and molecular level. inoculation decreased disease severity by significant reduction of seed infection level. symbiont enhanced yield through increased seed fresh and dry weights based on better seed filling. inoculation also induced changes in seed proteome and metabolome involved in enhanced resistance level against . Besides increased redox and cell wall adjustments light is shed on the role of late embryogenesis abundant proteins and metabolites such as the seed triterpenoid Soyasapogenol. The results of this study open new insights into the significance of symbiotic interactions for crop yield, health and seed quality enhancement and reveal new metabolite candidates involved in pathogen resistance.
在豌豆中，壳二孢叶枯病是由[病原菌名称未给出]引起的最常见的真菌病害之一。尽管豌豆品种具有高度多样性，但迄今为止仅培育出了很少的抗性品种，这仍然导致了谷物产量的显著损失。作为促进植物生长的内共生体，根瘤菌是与豌豆植株建立共生关系的主要伙伴。[根瘤菌名称未给出]作为豆类种子质量和数量提升的有效氮源所起的关键作用，与可持续农业和粮食安全计划相契合。除了这些促进生长的作用外，[根瘤菌名称未给出]共生关系已被证明对植物免疫系统具有引发作用，可增强对环境干扰的抗性。这是第一项综合研究，通过代谢组学和蛋白质组学分析，研究[根瘤菌菌株名称未给出]对盆栽豌豆（[豌豆品种名称未给出]）中具有两种不同[病原菌名称未给出]抗性水平的品种的种子表型质量、数量和真菌病害的影响。此外，还在形态学和分子水平上评估了病原菌对种子数量组成和质量的影响。[根瘤菌名称未给出]接种通过显著降低种子感染水平降低了病害严重程度。[根瘤菌名称未给出]共生体通过更好的种子充实增加种子鲜重和干重从而提高产量。[根瘤菌名称未给出]接种还诱导了种子蛋白质组和代谢组的变化，这些变化与增强对[病原菌名称未给出]的抗性水平有关。除了氧化还原和细胞壁调节增加外，还揭示了晚期胚胎发生丰富蛋白和代谢物如种子三萜大豆皂草醇的作用。本研究结果为共生[根瘤菌名称未给出]相互作用对作物产量、健康和种子质量提升的重要性提供了新的见解，并揭示了参与病原菌抗性的新代谢物候选物。
