Liu Dong, Liu Zeng-Shan, Hu Pan, Cai Ling, Fu Bao-Quan, Li Yan-Song, Lu Shi-Ying, Liu Nan-Nan, Ma Xiao-Long, Chi Dan, Chang Jiang, Shui Yi-Ming, Li Zhao-Hui, Ahmad Waqas, Zhou Yu, Ren Hong-Lin
Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, China.
Marine Environment Management and Development Strategy Research Center, The Third Institute of Oceanography, National Bureau of Oceanography, Xiamen 361005, China.
Vet Microbiol. 2016 Apr 15;186:126-38. doi: 10.1016/j.vetmic.2016.02.018. Epub 2016 Feb 26.
Acinetobacter baumannii is a Gram-negative bacillus that causes nosocomial infections, such as bacteremia, pneumonia, and meningitis and urinary tract and wound infections. In the present study, the surface antigen protein 1 (SurA1) gene of A. baumannii strain CCGGD201101 was identified, cloned and expressed, and then its roles in fitness and virulence were investigated. Virulence was observed in the human lung cancer cell lines A549 and HEp-2 at one week after treatment with recombinant SurA1. One isogenic SurA1 knock-out strain, GR0015, which was derived from the A. baumannii strain CCGGD201101 isolated from diseased chicks in a previous study, highlighted the effect of SurA1 on fitness and growth. Its growth rate in LB broth and killing activity in human sera were significantly decreased compared with strain CCGGD201101. In the Galleria mellonella insect model, the isogenic SurA1 knock-out strain exhibited a lower survival rate and decreased dissemination. These results suggest that SurA1 plays an important role in the fitness and virulence of A. baumannii.
鲍曼不动杆菌是一种革兰氏阴性杆菌，可引起医院感染，如菌血症、肺炎、脑膜炎以及泌尿道和伤口感染。在本研究中，对鲍曼不动杆菌菌株CCGGD201101的表面抗原蛋白1（SurA1）基因进行了鉴定、克隆和表达，然后研究了其在适应性和毒力方面的作用。在用重组SurA1处理一周后，在人肺癌细胞系A549和HEp-2中观察到了毒力。一种同基因SurA1敲除菌株GR0015，它源自先前一项研究中从患病雏鸡分离出的鲍曼不动杆菌菌株CCGGD201101，突出了SurA1对适应性和生长的影响。与菌株CCGGD201101相比，它在LB肉汤中的生长速率和在人血清中的杀伤活性显著降低。在大蜡螟昆虫模型中，同基因SurA1敲除菌株的存活率较低且传播减少。这些结果表明，SurA1在鲍曼不动杆菌的适应性和毒力中起着重要作用。
