Children's Health Research Institute & Lawson Health Research Institute, Departments of Obstetrics & Gynaecology and Physiology & Pharmacology, Western University, 800 Commissioners Rd. E., London, Ontario N6C 2V5, Canada.
Children's Health Research Institute & Lawson Health Research Institute, Departments of Obstetrics & Gynaecology and Physiology & Pharmacology, Western University, 800 Commissioners Rd. E., London, Ontario N6C 2V5, Canada.
Environ Toxicol Pharmacol. 2016 Apr;43:203-8. doi: 10.1016/j.etap.2016.03.014. Epub 2016 Mar 17.
The present study sought to determine if prenatal exposure to bisphenol A (BPA) alters adrenal steroidogenesis in adult offspring. Pregnant mice were exposed to BPA (25mg BPA/kg food pellet) via diet from day 7 to the end of pregnancy. At eight weeks of age, offsprings were sacrificed, blood samples and adrenal glands were collected for hormone assays and western blot analysis, respectively. We found that: (1) BPA increased adrenal gland weight in both males and females; (2) although BPA elevated plasma corticosterone levels in both sexes, it stimulated the expression of StAR and cyp11A1, the two rate-limiting factors in the steroidogenic pathway, only in female adrenal glands; and interestingly (3) BPA did not alter plasma ACTH levels or adrenal expression of the key steroidogenic transcription factor SF-1 in either sex. Taken together, the present study provides novel insights into the long-term consequences of developmental BPA exposure on adrenal steroidogenesis.
本研究旨在探讨产前暴露于双酚 A（BPA）是否会改变成年后代的肾上腺类固醇生成。怀孕小鼠从第 7 天到妊娠结束通过饮食暴露于 BPA（25mg BPA/kg 食物丸）。在 8 周龄时，处死后代，收集血液样本和肾上腺，分别用于激素测定和 Western blot 分析。我们发现：（1）BPA 增加了雄性和雌性的肾上腺重量；（2）尽管 BPA 增加了两性的血浆皮质酮水平，但它仅刺激了雌性肾上腺中类固醇生成途径的两个限速因子 StAR 和 cyp11A1 的表达；有趣的是（3）BPA 没有改变两性的血浆 ACTH 水平或关键类固醇生成转录因子 SF-1 的肾上腺表达。总之，本研究为发育过程中 BPA 暴露对肾上腺类固醇生成的长期后果提供了新的见解。
