Costa Luciana Fachini, Pessoa Moisés Sena, Guimarães Laís Bitencourt, Faria Anne Karoene Silva, Morão Rodrigo Pereira, Mol Juliana Pinto da Silva, Garcia Luize Néli Nunes, Almeida Anna Christina, Gouveia Aurora Maria Guimarães, Silva Marcos Xavier, Paixão Tatiane Alves, Santos Renato Lima
Departamento de Patologia, Instituto de Ciências Biológicas da Universidade Federal de Minas Gerais, CEP 31.270-901, Belo Horizonte, MG, Brazil.
Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária da Universidade Federal Minas Gerais, CEP 31.270-901, Belo Horizonte, MG, Brazil.
BMC Res Notes. 2016 Mar 26;9:190. doi: 10.1186/s13104-016-1998-2.
Brucella ovis infection is one of the leading causes of sub fertility and infertility in ovine, been characterized mainly by epididymitis, orchitis and testicular atrophy in rams. This study aimed to determine the frequency of B. ovis positivity in rams and goats flocks in the State of Minas Gerais, Brazil, by agarose gel immunodiffusion (AGID), ELISA, Rose Bengal, PCR and bacteriological isolation as diagnostic tools.
Serum and urine samples were collected from properties with sheep or goat flocks, or from properties with mixed flock. Out of 50 sheep flocks, 6% (3/50) were seropositive by AGID while 4% (2/50) were positive by urine PCR for B. ovis. Out of five goat farms, 20% (1/5) were seropositive for B. ovis by AGID. Mixed flock farms had 11.1% (2/18) of positivity by AGID. By ELISA, 19.5% (8/41) of sheep properties and 61.1% (11/18) of the properties with mixed flocks were positive for B. ovis. No samples were positive in the test of Rose Bengal, ruling out exposure to smooth LPS Brucella species (particularly Brucella melitensis) and indicating that the positive in the ELISA was associated with Brucella spp. LPS rough (presumably B. ovis). No urine sample from sheep or goat was positive by bacteriological isolation.
Our results demonstrate serologic or molecular evidence of B. ovis infection in several rams and billy goats from meso-regions of the State of Minas Gerais, Brazil. Also, this study report the indirect ELISA as an important tool for the diagnosis of B. ovis infection, as indirect ELISA in this study demonstrated to be the most sensitive diagnostic method adopted.
绵羊种布鲁氏菌感染是绵羊亚生育力和不育的主要原因之一，主要特征为公绵羊附睾炎、睾丸炎和睾丸萎缩。本研究旨在通过琼脂糖凝胶免疫扩散法（AGID）、酶联免疫吸附测定（ELISA）、玫瑰红试验、聚合酶链反应（PCR）和细菌分离作为诊断工具，确定巴西米纳斯吉拉斯州公绵羊和山羊群中绵羊种布鲁氏菌阳性的频率。
从有绵羊或山羊群的养殖场或有混合群的养殖场采集血清和尿液样本。在50个绵羊群中，AGID血清学阳性率为6%（3/50），尿液PCR检测绵羊种布鲁氏菌阳性率为4%（2/50）。在5个山羊养殖场中，AGID检测绵羊种布鲁氏菌血清学阳性率为20%（1/5）。混合群养殖场AGID阳性率为11.1%（2/18）。ELISA检测显示，19.5%（8/41）的绵羊养殖场和61.1%（11/18）的混合群养殖场绵羊种布鲁氏菌呈阳性。玫瑰红试验中没有样本呈阳性，排除了接触光滑脂多糖布鲁氏菌属物种（特别是马尔他布鲁氏菌）的可能性，并表明ELISA检测呈阳性与粗糙脂多糖布鲁氏菌属（推测为绵羊种布鲁氏菌）有关。绵羊或山羊的尿液样本经细菌分离均未呈阳性。
我们的结果证明，在巴西米纳斯吉拉斯州中部地区的几只公绵羊和公山羊中存在绵羊种布鲁氏菌感染的血清学或分子证据。此外，本研究报告间接ELISA是诊断绵羊种布鲁氏菌感染的重要工具，因为本研究中的间接ELISA是所采用的最敏感的诊断方法。
