Stricto Sensu Program in Animal Health and Production, Universidade Pitágoras Unopar, Arapongas, Parana State, Brazil.
J Vet Diagn Invest. 2022 Jan;34(1):164-166. doi: 10.1177/10406387211053589. Epub 2021 Oct 26.
is the causative agent of ovine brucellosis, which is an important infectious disease in sheep farming worldwide and is responsible for economic losses because of its negative effect on the reproductive system of rams and ewes. Serologic tests are the main tools for detection of infection; however, these tests commonly yield a high frequency of false-negative results. We compared 2 serologic tests, agar gel immunodiffusion (AGID) and ELISA, for the detection of anti- antibodies in naturally infected sheep. Of the 728 serum samples analyzed, 0.3% were positive by AGID and 9.2% by ELISA. Positive results were obtained for different animals and flocks. There was no statistical difference between the detection frequency of the 2 methods ( = 0.674), and the kappa test indicated low concordance (κ = 0.005). The lack of agreement between results obtained using AGID and ELISA, associated with the absence of clinical signs, makes it difficult to detect ovine brucellosis efficiently, and demonstrates the need for effective tests for the definitive detection of infection.
绵羊布鲁氏菌病是由布鲁氏菌引起的，是一种重要的绵羊养殖传染病，会对公羊和母羊的生殖系统造成负面影响，导致严重的经济损失。血清学检测是感染检测的主要手段，但这些检测通常会出现较高的假阴性结果。我们比较了琼脂凝胶免疫扩散（AGID）和 ELISA 这两种血清学检测方法，用于检测自然感染绵羊的抗抗体。在分析的 728 份血清样本中，AGID 检测的阳性率为 0.3%，ELISA 检测的阳性率为 9.2%。不同动物和羊群都出现了阳性结果。两种方法的检测频率无统计学差异（=0.674），kappa 检验表明一致性低（κ=0.005）。AGID 和 ELISA 检测结果不一致，且无临床症状，这使得绵羊布鲁氏菌病的有效检测变得困难，表明需要有效的检测方法来明确布鲁氏菌感染的诊断。
