Duong Tuan A, de Beer Z Wilhelm, Wingfield Brenda D, Wingfield Michael J
Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa.
Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa.
Fungal Biol. 2016 Apr;120(4):538-546. doi: 10.1016/j.funbio.2016.01.001. Epub 2016 Jan 21.
Species of Leptographium sensu lato are sap-stain fungi vectored by bark beetles and some species cause or are associated with tree diseases. Sexual states have been reported for more than 30 species in this group and these have been treated in the sexual genus Grosmannia. No sexual state is known for at least 59 additional species and these reside in the genus Leptographium. The discovery of sexual states for species of Leptographium relies mainly on the presence of fruiting bodies on host tissue at the time of isolation and/or intensive laboratory mating studies, which commonly have low levels of success. We developed mating-type markers to study sexual compatibility of species in Leptographium sensu lato. Using these markers, it was possible to identify mating types for 42 species and to determine thallism in many species for the first time. Surprisingly, the results showed that heterothallic and putatively heterothallic species are abundant (39 out of 42 species) in Leptographium sensu lato, and only three species were confirmed to be homothallic. The mating type markers developed in this study will be useful for future studies concerning mating type and sexual compatibility of species in this genus.
广义细顶束梗孢属的物种是由小蠹虫传播的变色真菌，一些物种会引发或与树木疾病有关。该类群中已有30多个物种报道了有性态，这些有性态被归入有性属格罗斯曼霉属。至少还有59个物种尚无有性态的报道，它们归属于细顶束梗孢属。细顶束梗孢属物种有性态的发现主要依赖于分离时在寄主组织上是否存在子实体和/或密集的实验室交配研究，但这些方法通常成功率较低。我们开发了交配型标记来研究广义细顶束梗孢属物种的性亲和性。利用这些标记，首次有可能鉴定出42个物种的交配型，并确定许多物种的同宗配合情况。令人惊讶的是，结果表明，广义细顶束梗孢属中异宗配合和假定异宗配合的物种很丰富（42个物种中有39个），只有3个物种被确认为同宗配合。本研究中开发的交配型标记将有助于今后对该属物种的交配型和性亲和性的研究。
