Kováčik Jozef, Klejdus Bořivoj, Štork František, Hedbavny Josef, Bačkor Martin
Department of Botany, Institute of Biology and Ecology, Faculty of Science, P. J. Šafárik University, Mánesova 23, 041 67 Košice, Slovak RepublicDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech RepublicDepartment of Botany, Institute of Biology and Ecology, Faculty of Science, P. J. Šafárik University, Mánesova 23, 041 67 Košice, Slovak RepublicDepartment of Chemistry and Biochemistry, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech RepublicDepartment of Botany, Institute of Biology and Ecology, Faculty of Science, P. J. Šafárik University, Mánesova 23, 041 67 Košice, Slovak Republic.
J Phycol. 2011 Oct;47(5):1044-9. doi: 10.1111/j.1529-8817.2011.01027.x. Epub 2011 Jul 21.
Effect of methyl jasmonate (MeJA) in the concentrations of 10 or 100 μM on selected physiological parameters in Scenedesmus quadricauda (Turp.) Bréb. after 24 h of exposure was studied. Results were compared with the application of general toxic metal (cadmium, Cd) to identify MeJA-specific responses. Accumulation of reactive oxygen species (ROS; hydrogen peroxide and superoxide) was the most elevated by 10 μM MeJA and 100 μM Cd, while total chls showed decrease (Cd) and increase (MeJA) in these variants. The amount of carotenoids and cell viability were affected neither by MeJA nor by Cd application. The sum of free amino acids was considerably elevated by 10 μM Cd (increase in histidine, threonine, arginine, leucine, and lysine mainly) but depleted by 100 μM MeJA (14 from 17 compounds decreased), while accumulation of soluble proteins was unaffected by Cd and enhanced by MeJA. Cadmium application reduced the amount of Ca and also Mg in the case of 100 μM Cd, while MeJA had no effect on the content of mineral nutrients. Total Cd content reached 557 and 1,334 μg · g(-1) dry weight (dwt) in 10 and 100 μM Cd variant, respectively. Intracellular Cd uptake was ca. 55% from total Cd content in both Cd variants. The present findings are discussed in the context of the available literature, and possible explanations are suggested.
研究了10或100μM浓度的茉莉酸甲酯（MeJA）对四尾栅藻（Scenedesmus quadricauda (Turp.) Bréb.）在暴露24小时后选定生理参数的影响。将结果与一般有毒金属（镉，Cd）的应用进行比较，以确定MeJA特异性反应。活性氧（ROS；过氧化氢和超氧化物）的积累在10μM MeJA和100μM Cd处理下升高最为明显，而在这些变体中，总叶绿素含量呈现下降（Cd处理）和增加（MeJA处理）。类胡萝卜素含量和细胞活力不受MeJA或Cd处理的影响。游离氨基酸总量在10μM Cd处理下显著升高（主要是组氨酸、苏氨酸、精氨酸、亮氨酸和赖氨酸增加），但在100μM MeJA处理下减少（17种化合物中的14种减少），而可溶性蛋白质的积累不受Cd影响，但受MeJA增强。施用镉会降低钙的含量，在100μM Cd处理下还会降低镁的含量，而MeJA对矿质营养元素的含量没有影响。在10和100μM Cd变体中，总镉含量分别达到557和1334μg·g(-1)干重（dwt）。在两种镉变体中，细胞内镉的吸收量约占总镉含量的55%。本文结合现有文献对研究结果进行了讨论，并提出了可能的解释。
