Farhat Nèjia, Rabhi Mokded, Falleh Hanen, Jouini Jihène, Abdelly Chedly, Smaoui Abderrazak
Laboratory of Extremophile Plants (LPE), Biotechnology Center of Borj-Cedria, P.O. Box 901, Hammam-Lif 2050, Tunisia.
J Phycol. 2011 Oct;47(5):1072-7. doi: 10.1111/j.1529-8817.2011.01036.x. Epub 2011 Aug 19.
Dunaliella salina (Dunal) Teodor, when treated over 25 d with a wide range of NaCl salinities (0.6-4.5 M), showed its maximal growth potentialities at 1.5-3.0 M NaCl and was able to survive even at 4.5 M NaCl. Sodium concentrations increased significantly at the supraoptimal salinities, reaching up to 5 mmol · g(-1) dry weight (dwt) at 4.5 M NaCl. Interestingly, ability of D. salina to take up essential mineral nutrients was not impaired by increased salinity. As for growth, chl concentrations were maximal in the 1.5-3.0 M NaCl range. Interestingly, carotenoid concentrations increased with the increasing salinity. The highest values of total antioxidant activity (5.2-6.9 mg gallic acid equivalents [GAE] · g(-1) dwt), antiradical activity, and reducing power were measured at 1.5-3.0 M NaCl. As a whole, these results showed that at 1.5-3.0 M NaCl, D. salina produce appreciable antioxidant level. But, once it reaches its growth maximum, a salt addition up to 4.5 M could enhance its carotenoid yield.
盐生杜氏藻（杜纳尔）特奥多尔，在25天以上的时间里用多种氯化钠盐度（0.6 - 4.5 M）处理时，在1.5 - 3.0 M氯化钠浓度下展现出最大生长潜力，甚至在4.5 M氯化钠浓度下也能存活。在超适宜盐度下，钠浓度显著增加，在4.5 M氯化钠时达到高达5 mmol·g(-1)干重（dwt）。有趣的是，盐生杜氏藻吸收必需矿物质营养的能力并未因盐度增加而受损。至于生长，叶绿素浓度在1.5 - 3.0 M氯化钠范围内最高。有趣的是，类胡萝卜素浓度随盐度增加而升高。在1.5 - 3.0 M氯化钠时测得总抗氧化活性（5.2 - 6.9 mg没食子酸当量[GAE]·g(-1) dwt）、抗自由基活性和还原能力的最高值。总体而言，这些结果表明，在1.5 - 3.0 M氯化钠时，盐生杜氏藻产生可观的抗氧化水平。但是，一旦达到生长最大值，添加高达4.5 M的盐可以提高其类胡萝卜素产量。
