多克隆激活剂对血细胞及恶性乳腺癌细胞产生细胞因子的影响。

Effect of polyclonal activators on cytokine production by blood cells and by malignant breast cancer cells.

作者信息

Kunts T A, Karpukhina K V, Mikhaylova E S, Marinkin I O, Varaksin N A, Autenshlyus A I, Lyakhovich V V

机构信息

Research Center for Molecular Biology and Biophysics, Siberian Branch, Russian Academy of Medical Sciences, Novosibirsk, Russia.

Novosibirsk State Medical University, Ministry of Health, Novosibirsk, Russia.

出版信息

Dokl Biol Sci. 2016;466:45-7. doi: 10.1134/S0012496616010026. Epub 2016 Mar 30.

DOI:10.1134/S0012496616010026

PMID:27021370

Abstract

The production of cytokines by peripheral blood cells and biopsy specimens of tumors stimulated by polyclonal activators (PAs) was evaluated in 34 patients with invasive ductal breast carcinoma using enzyme-linked immunosorbent assay (ELISA). Positive correlation between the stimulation index of polyclonal activators (SIPA) for IL-18 production by the tumor and the relative content of poorly differentiated cells was revealed. The latter, in turn, was positively correlated with the numbers of normal and pathologic mitoses and the degree of malignancy. Cancer cells can produce IL-18, which is involved in the process of angiogenesis, stimulates invasion and metastasis. Decrease in SIPA for the production of IL-6 and GCSF by peripheral blood cells could serve as an indicator of malignant progression in invasive ductal breast carcinoma.

摘要

采用酶联免疫吸附测定法（ELISA），对34例浸润性导管癌患者外周血细胞和肿瘤活检标本经多克隆激活剂（PAs）刺激后细胞因子的产生情况进行了评估。结果显示，肿瘤产生白细胞介素-18（IL-18）的多克隆激活剂刺激指数（SIPA）与低分化细胞的相对含量呈正相关。而低分化细胞的相对含量又与正常及病理性有丝分裂数量和恶性程度呈正相关。癌细胞可产生IL-18，其参与血管生成过程，刺激侵袭和转移。外周血细胞产生IL-6和粒细胞集落刺激因子（GCSF）的SIPA降低可作为浸润性导管癌恶性进展的一个指标。