Sarinont Thapanut, Amano Takaaki, Attri Pankaj, Koga Kazunori, Hayashi Nobuya, Shiratani Masaharu
Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul 139-701, South Korea.
Arch Biochem Biophys. 2016 Sep 1;605:129-40. doi: 10.1016/j.abb.2016.03.024. Epub 2016 Mar 26.
In this work, we have studied the action of dielectric barrier discharge (DBD) plasma irradiation using various feeding gases on seeds of Raphanus sativus L. and analysis their growth. Our experimental data shows that Air, O2, and NO(10%)+N2 feeding gases plasma irradiation enhanced plant growth, whereas N2, He and Ar feeding gases plasma irradiation had little influence on plant growth. Moreover, humid air plasma irradiation was more effective in growth enhancement than dry one. More than 2.3 times faster growth was observed by 3 min air plasma irradiation with 40-90% relative humidity. The reactive species generated by plasma in gas phase were detected using optical emission spectroscopy and in liquid phase by electron spin resonance (ESR) spectroscopy. We concluded that OH and O radicals were key species for plant growth enhancement.
在本研究中,我们研究了使用各种供气气体的介质阻挡放电(DBD)等离子体辐照对萝卜种子的作用,并分析了它们的生长情况。我们的实验数据表明,空气、氧气和NO(10%)+氮气供气气体的等离子体辐照促进了植物生长,而氮气、氦气和氩气供气气体的等离子体辐照对植物生长影响很小。此外,潮湿空气等离子体辐照在促进生长方面比干燥空气更有效。在相对湿度为40-90%的条件下,3分钟空气等离子体辐照使生长速度加快了2.3倍以上。利用光发射光谱法检测了气相中等离子体产生的活性物种,并通过电子自旋共振(ESR)光谱法检测了液相中的活性物种。我们得出结论,OH和O自由基是促进植物生长的关键物种。