Department of Textile engineering, Isfahan university of technology, Isfahan 84156-83111, Iran.
Department of Textile engineering, Isfahan university of technology, Isfahan 84156-83111, Iran.
Mater Sci Eng C Mater Biol Appl. 2016 Jun;63:106-16. doi: 10.1016/j.msec.2016.02.051. Epub 2016 Feb 19.
Fabrication of bioactive scaffolds is one of the most promising strategies to reconstruct the infarcted myocardium. In this study, we synthesized polyester urethane urea (PEUU), further blended it with gelatin and fabricated PEUU/G nanofibrous scaffolds. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC) and X-ray diffraction were used for the characterization of the synthesized PEUU and properties of nanofibrous scaffolds were evaluated using scanning electron microscopy (SEM), ATR-FTIR, contact angle measurement, biodegradation test, tensile strength analysis and dynamic mechanical analysis (DMA). In vitro biocompatibility studies were performed using cardiomyocytes. DMA analysis showed that the scaffolds could be reshaped with cyclic deformations and might remain stable in the frequencies of the physiological activity of the heart. On the whole, our study suggests that aligned PEUU/G 70:30 nanofibrous scaffolds meet the required specifications for cardiac tissue engineering and could be used as a promising construct for myocardial regeneration.
生物活性支架的制备是重建梗死心肌最有前途的策略之一。在本研究中，我们合成了聚酯氨酯脲（PEUU），进一步将其与明胶混合，制备了 PEUU/G 纳米纤维支架。采用衰减全反射傅里叶变换红外光谱（ATR-FTIR）、差示扫描量热法（DSC）和 X 射线衍射对合成的 PEUU 进行了表征，并采用扫描电子显微镜（SEM）、ATR-FTIR、接触角测量、生物降解试验、拉伸强度分析和动态力学分析（DMA）对纳米纤维支架的性能进行了评估。采用心肌细胞进行了体外生物相容性研究。DMA 分析表明，支架可以通过周期性变形重塑，并且在心脏生理活动的频率下可能保持稳定。总的来说，我们的研究表明，定向排列的 PEUU/G70:30 纳米纤维支架符合心脏组织工程的要求，可以作为心肌再生的有前途的构建体。
