Nanomedicine and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Int J Nanomedicine. 2011;6:2133-41. doi: 10.2147/IJN.S24312. Epub 2011 Sep 27.
In this study, gelatin was blended with polyglycolic acid (PGA) at different ratios (0, 10, 30, and 50 wt%) and electrospun. The morphology and structure of the scaffolds were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and differential scanning calorimetry. The mechanical properties were also measured by the tensile test. Furthermore, for biocompatibility assessment, human umbilical vein endothelial cells and human umbilical artery smooth muscle cells were cultured on these scaffolds, and cell attachment and viability were evaluated.
PGA with 10 wt% gelatin enhanced the endothelial cells whilst PGA with 30 wt% gelatin increased smooth muscle cell adhesion, penetration, and viability compared with the other scaffold blends. Additionally, with the increase in gelatin content, the mechanical properties of the scaffolds were improved due to interaction between PGA and gelatin, as revealed by Fourier transform infrared spectroscopy and differential scanning calorimetry.
Incorporation of gelatin improves the biological and mechanical properties of PGA, making promising scaffolds for vascular tissue engineering.
本研究将明胶与聚乙醇酸（PGA）以不同比例（0、10、30 和 50wt%）混合并进行静电纺丝。通过扫描电子显微镜、傅里叶变换红外光谱和差示扫描量热法对支架的形态和结构进行了表征。通过拉伸试验测量了机械性能。此外，为了进行生物相容性评估，将人脐静脉内皮细胞和人脐动脉平滑肌细胞培养在这些支架上，评估细胞黏附性和活力。
与其他支架混合物相比，含有 10wt%明胶的 PGA 增强了内皮细胞，而含有 30wt%明胶的 PGA 增加了平滑肌细胞的黏附、渗透和活力。此外，随着明胶含量的增加，由于 PGA 和明胶之间的相互作用，支架的机械性能得到了改善，这一点通过傅里叶变换红外光谱和差示扫描量热法得到了揭示。
明胶的加入改善了 PGA 的生物和机械性能，使其成为有前途的血管组织工程支架。
