Rodríguez-Cerezo E, Moya A, García-Arenal F
Departamento de Patología Vegetal, E.T.S.I. Agrónomos, Madrid, Spain.
J Virol. 1989 May;63(5):2198-203. doi: 10.1128/JVI.63.5.2198-2203.1989.
The RNA genomes of 26 isolates of pepper mild mottle virus were compared by their RNase T1 fingerprints. Twenty-three isolates came from epidemic outbreaks in greenhouse-grown peppers in Almería (southeastern Spain) from 1983 to 1987; three other isolates, from 1980, came from Sicily (Italy) and Zaragoza (central Spain). The 26 fingerprints can be classified into 10 different types; nucleotide substitution rates show them to be very similar. Cluster and cladistic analyses group types corresponding to the Almería isolates separate from those of 1980. Intraannual and interannual nucleotide differences were estimated. An evolutionary model for pepper mild mottle virus built on these data indicates a highly stable population, maintaining its diversity through time, with a main prevailing haplotype from which closely related variants arise that do not replace it. This high stability could be due to strong functional constraints on variation, as suggested by the high proportion of invariant versus polymorphic sites in fingerprints.
通过RNase T1指纹图谱对26株辣椒轻斑驳病毒的RNA基因组进行了比较。其中23株分离自1983年至1987年西班牙东南部阿尔梅里亚温室种植辣椒的疫情爆发；另外3株分离自1980年，分别来自意大利西西里岛和西班牙中部的萨拉戈萨。这26个指纹图谱可分为10种不同类型；核苷酸替代率表明它们非常相似。聚类分析和分支分析将阿尔梅里亚分离株对应的类型与1980年的分离株区分开来。估计了年内和年际核苷酸差异。基于这些数据构建的辣椒轻斑驳病毒进化模型表明，该病毒群体高度稳定，随时间推移保持其多样性，存在一个主要的优势单倍型，与之密切相关的变体由此产生，但不会取代它。这种高稳定性可能是由于对变异的强大功能限制，指纹图谱中不变位点与多态性位点的高比例表明了这一点。
