Xie Hui, Sun Li, Zhang Liming, Liu Teng, Chen Li, Zhao Aiqi, Lei Qian, Gao Fei, Zou Ping, Li Qiubai, Guo An-Yuan, Chen Zhichao, Wang Hongxiang
Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Department of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
Stem Cells Int. 2016;2016:6493241. doi: 10.1155/2016/6493241. Epub 2016 Mar 6.
Mesenchymal stem cells (MSCs) are known to support the characteristic properties of hematopoietic stem and progenitor cells (HSPCs) in the bone marrow hematopoietic microenvironment. MSCs are used in coculture systems as a feeder layer for the ex vivo expansion of umbilical cord blood (CB) to increase the relatively low number of HSPCs in CB. Findings increasingly suggest that MSC-derived microvesicles (MSC-MVs) play an important role in the biological functions of their parent cells. We speculate that MSC-MVs may recapitulate the hematopoiesis-supporting effects of their parent cells. In the current study, we found MSC-MVs containing microRNAs that are involved in the regulation of hematopoiesis. We also demonstrated that MSC-MVs could improve the expansion of CB-derived mononuclear cells and CD34(+) cells and generate a greater number of primitive progenitor cells in vitro. Additionally, when MSC-MVs were added to the CB-MSC coculture system, they could improve the hematopoiesis-supporting effects of MSCs. These findings highlight the role of MSC-MVs in the ex vivo expansion of CB, which may offer a promising therapeutic approach in CB transplantation.
间充质干细胞（MSCs）已知可在骨髓造血微环境中支持造血干细胞和祖细胞（HSPCs）的特性。在共培养系统中，MSCs被用作饲养层，用于脐血（CB）的体外扩增，以增加CB中相对较少的HSPCs数量。越来越多的研究结果表明，间充质干细胞衍生的微泡（MSC-MVs）在其母细胞的生物学功能中发挥重要作用。我们推测MSC-MVs可能重现其母细胞的造血支持作用。在本研究中，我们发现MSC-MVs含有参与造血调控的微小RNA。我们还证明，MSC-MVs可以改善CB来源的单个核细胞和CD34(+)细胞的扩增，并在体外产生更多的原始祖细胞。此外，当将MSC-MVs添加到CB-MSC共培养系统中时，它们可以改善MSCs的造血支持作用。这些发现突出了MSC-MVs在CB体外扩增中的作用，这可能为CB移植提供一种有前景的治疗方法。
