乌头汤对胶原诱导性关节炎大鼠DNA甲基化和组蛋白修饰的影响
Effects of Wutou Decoction on DNA Methylation and Histone Modifications in Rats with Collagen-Induced Arthritis.
作者信息
Liu Ya-Fei, Wen Cai-Yu-Zhu, Chen Zhe, Wang Yu, Huang Ying, Hu Yong-Hong, Tu Sheng-Hao
机构信息
Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, Hubei 430030, China; Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe East Road, Zhengzhou, Henan 450052, China.
Hubei University of Chinese Medicine, 1 Huangjiahu West Road, Wuhan, Hubei 430065, China.
出版信息
Evid Based Complement Alternat Med. 2016;2016:5836879. doi: 10.1155/2016/5836879. Epub 2016 Mar 6.
Background. Wutou decoction (WTD) has been wildly applied in the treatment of rheumatoid arthritis and experimental arthritis in rats for many years. Epigenetic deregulation is associated with the aetiology of rheumatoid arthritis; however, the effects of WTD on epigenetic changes are unclear. This study is set to explore the effects of WTD on DNA methylation and histone modifications in rats with collagen-induced arthritis (CIA). Methods. The CIA model was established by the stimulation of collagen and adjuvant. The knee synovium was stained with hematoxylin and eosin. The DNA methyltransferase 1 (DNMT1) and methylated CpG binding domain 2 (MBD2) expression of peripheral blood mononuclear cells (PBMCs) were determined by Real-Time PCR. The global DNA histone H3-K4/H3-K27 methylation and total histones H3 and H4 acetylation of PBMCs were detected. Results. Our data demonstrated that the DNMT1 mRNA expression was significantly lowered in group WTD compared to that in group CIA (P < 0.05). The DNA methylation level was significantly reduced in group WTD compared to that in group CIA (P < 0.05). Moreover, H3 acetylation of PBMCs was overexpressed in WTD compared with CIA (P < 0.05). Conclusions. WTD may modulate DNA methylation and histone modifications, functioning as anti-inflammatory potential.
背景。乌头汤（WTD）多年来一直广泛应用于类风湿关节炎的治疗及大鼠实验性关节炎的研究。表观遗传失调与类风湿关节炎的病因相关；然而，乌头汤对表观遗传变化的影响尚不清楚。本研究旨在探讨乌头汤对胶原诱导性关节炎（CIA）大鼠DNA甲基化和组蛋白修饰的影响。方法。通过胶原和佐剂刺激建立CIA模型。膝关节滑膜进行苏木精-伊红染色。采用实时荧光定量PCR检测外周血单核细胞（PBMCs）中DNA甲基转移酶1（DNMT1）和甲基化CpG结合域2（MBD2）的表达。检测PBMCs的全基因组DNA组蛋白H3-K4/H3-K27甲基化及总组蛋白H3和H4乙酰化水平。结果。我们的数据表明，与CIA组相比，WTD组的DNMT1 mRNA表达显著降低（P < 0.05）。与CIA组相比，WTD组的DNA甲基化水平显著降低（P < 0.05）。此外，与CIA组相比，WTD组PBMCs的H3乙酰化水平过表达（P < 0.05）。结论。乌头汤可能调节DNA甲基化和组蛋白修饰，具有抗炎潜能。
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