通过夹层光刻法制备抗蛋白质污染的聚乙二醇微流控芯片电泳
Fabrication of anti-protein-fouling poly(ethylene glycol) microfluidic chip electrophoresis by sandwich photolithography.
作者信息
Cong Hailin, Xu Xiaodan, Yu Bing, Liu Huwei, Yuan Hua
机构信息
College of Materials Science and Engineering, Qingdao University , Qingdao 266071, China.
Beijing National Laboratory for Molecular Sciences (BNLMS), College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
出版信息
Biomicrofluidics. 2016 Jul 19;10(4):044106. doi: 10.1063/1.4959239. eCollection 2016 Jul.
Microfluidic chip electrophoresis (MCE) is a powerful separation tool for biomacromolecule analysis. However, adsorption of biomacromolecules, particularly proteins onto microfluidic channels severely degrades the separation performance of MCE. In this paper, an anti-protein-fouling MCE was fabricated using a novel sandwich photolithography of poly(ethylene glycol) (PEG) prepolymers. Photopatterned microchannel with a minimum resolution of 10 μm was achieved. After equipped with a conventional online electrochemical detector, the device enabled baseline separation of bovine serum albumin, lysozyme (Lys), and cytochrome c (Cyt-c) in 53 s under a voltage of 200 V. Compared with a traditional polydimethylsiloxane MCE made by soft lithography, the PEG MCE made by the sandwich photolithography not only eliminated the need of a master mold and the additional modification process of the microchannel but also showed excellent anti-protein-fouling properties for protein separation.
微流控芯片电泳（MCE）是一种用于生物大分子分析的强大分离工具。然而，生物大分子，尤其是蛋白质在微流控通道上的吸附会严重降低MCE的分离性能。本文利用聚乙二醇（PEG）预聚物的新型三明治光刻技术制备了一种抗蛋白质污染的MCE。实现了最小分辨率为10μm的光图案化微通道。在配备传统的在线电化学检测器后，该装置在200V电压下53秒内实现了牛血清白蛋白、溶菌酶（Lys）和细胞色素c（Cyt-c）的基线分离。与通过软光刻制备的传统聚二甲基硅氧烷MCE相比，通过三明治光刻制备的PEG MCE不仅无需母模和微通道的额外改性过程，而且在蛋白质分离方面表现出优异的抗蛋白质污染性能。
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