Kubatka Peter, Kello Martin, Kajo Karol, Kruzliak Peter, Výbohová Desanka, Šmejkal Karel, Maršík Petr, Zulli Anthony, Gönciová Gabriela, Mojžiš Ján, Kapinová Andrea, Murin Radovan, Péč Martin, Adamkov Marián, Przygodzki Ronald M
a Department of Medical Biology , Jessenius Faculty of Medicine, Comenius University in Bratislava , Martin , Slovakia.
b Department of Pharmacology , Faculty of Medicine, P. J. Šafárik University , Košice , Slovakia.
Nutr Cancer. 2016 May-Jun;68(4):611-21. doi: 10.1080/01635581.2016.1154577. Epub 2016 Apr 4.
The effect of dietary administered young barley containing a mixture of phytochemicals to female rats for the prevention of N-methyl-N-nitrosourea-induced mammary carcinogenesis was evaluated. After carcinogen administration (14 wk), mammary tumors were removed and prepared for histopathological and immunohistochemical analysis. Moreover, in vitro evaluation of possible mechanisms in MCF-7 breast cancer cell line was performed. Barley (0.3%) demonstrated mild antitumor effect in mammary carcinogenesis, yet 3% barley did not further improve this effect. Immunohistochemical analysis of rat tumor cells in treated groups showed significant increase in caspase-3 expression and significant reduction in Ki67 expression. In addition, 3% barley significantly decreased dityrosine levels versus control. Barley in higher dose significantly decreased serum low-density lipoprotein-cholesterol in rats. In vitro studies showed that barley significantly decreased survival of MCF-7 cells in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and significantly decreased 5-bromo-20-deoxyuridine incorporation versus control. Barley prevented cell cycle progression and extended incubation with barley showed significant increase in the percentage of annexin V/propidium iodide-positive MCF-7 cells. Our results propose an antitumor effect for the mixture of phytochemicals present in young barley in a breast cancer model.
评估了饮食中给予含有多种植物化学物质混合物的嫩大麦对雌性大鼠预防N-甲基-N-亚硝基脲诱导的乳腺癌发生的影响。在给予致癌物（14周）后，切除乳腺肿瘤并进行组织病理学和免疫组织化学分析。此外，对MCF-7乳腺癌细胞系的可能机制进行了体外评估。大麦（0.3%）在乳腺癌发生中显示出轻度抗肿瘤作用，但3%的大麦并未进一步增强这种作用。治疗组大鼠肿瘤细胞的免疫组织化学分析显示，caspase-3表达显著增加，Ki67表达显著降低。此外，与对照组相比，3%的大麦显著降低了二酪氨酸水平。高剂量大麦显著降低了大鼠血清低密度脂蛋白胆固醇水平。体外研究表明，在3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐试验中，大麦显著降低了MCF-7细胞的存活率，与对照组相比，显著降低了5-溴-2'-脱氧尿苷掺入量。大麦阻止细胞周期进程，延长与大麦的孵育时间显示膜联蛋白V/碘化丙啶阳性MCF-7细胞的百分比显著增加。我们的结果表明，嫩大麦中存在的植物化学物质混合物在乳腺癌模型中具有抗肿瘤作用。
