Department of Macromolecular Science & Engineering, Case Western Reserve University , 2100 Adelbert Road, Cleveland, Ohio 44106, United States.
ACS Appl Mater Interfaces. 2016 Apr 13;8(14):8928-38. doi: 10.1021/acsami.6b00093. Epub 2016 Apr 4.
Antifouling surfaces are important for biomedical devices to prevent secondary infections and mitigate the effects of the foreign body response. Herein, we describe melt-coextruded poly(ε-caprolactone) (PCL) nanofiber mats grafted with antifouling polymers. Nonwoven PCL fiber mats are produced using a multilayered melt coextrusion process followed by high-pressure hydroentanglement to yield porous patches. The resulting fiber mats show submicrometer cross-sectional fiber dimensions and yield pore sizes that were nearly uniform, with a mean pore size of 1.6 ± 0.9 μm. Several antifouling polymers, including hydrophilic, zwitterionic, and amphipathic molecules, are grafted to the surface of the mats using a two-step procedure that includes photochemistry followed by the copper-catalyzed azide-alkyne cycloaddition reaction. Fiber mats are evaluated using separate adsorption tests for serum proteins and E. coli. The results indicate that poly(oligo(ethylene glycol) methyl ether methacrylate)-co-(trifluoroethyl methacrylate) (poly(OEGMEMA-co-TFEMA)) grafted mats exhibit approximately 85% less protein adhesion and 97% less E. coli adsorption when compared to unmodified PCL fibermats. In dynamic antifouling testing, the amphiphilic fluorous polymer surface shows the highest flux and highest rejection value of foulants. The work presented within has implications on the high-throughput production of antifouling microporous patches for medical applications.
抗污表面对于防止生物医学设备的二次感染和减轻异物反应的影响非常重要。在此，我们描述了通过熔融共挤出接枝有抗污聚合物的聚（ε-己内酯）（PCL）纳米纤维垫。使用多层熔融共挤出工艺生产无纺 PCL 纤维垫，然后通过高压水刺处理得到多孔垫。得到的纤维垫显示出亚微米级的横截面纤维尺寸和几乎均匀的孔径，平均孔径为 1.6±0.9μm。几种抗污聚合物，包括亲水、两性离子和两亲分子，通过两步法接枝到垫的表面，包括光化学和铜催化的叠氮-炔环加成反应。使用单独的血清蛋白和大肠杆菌吸附测试来评估纤维垫。结果表明，与未改性的 PCL 纤维垫相比，接枝有聚（聚乙二醇甲基醚甲基丙烯酸酯-co-三氟乙基甲基丙烯酸酯）（poly(OEGMEMA-co-TFEMA)）的垫的蛋白吸附量减少了约 85%，大肠杆菌吸附量减少了 97%。在动态抗污测试中，两亲氟聚合物表面显示出最高的通量和最高的污染物截留值。本工作对高通量生产用于医疗应用的抗污微孔垫具有重要意义。
