Department of Physics and Astronomy, University of Sheffield, Hounsfield Road, Sheffield, S3 7RH, UK.
Positron Emission Tomography Research Centre, University of Hull, Cottingham Road, Hull, HU6 7RX, UK and Department of Chemistry, University of Hull, Cottingham Road, Hull, HU6 7RX, UK.
Lab Chip. 2016 Apr 26;16(9):1605-16. doi: 10.1039/c6lc00099a.
The ability to detect radiation in microfluidic devices is important for the on-chip analysis of radiopharmaceuticals, but previously reported systems have largely suffered from various limitations including cost, complexity of fabrication, and insufficient sensitivity and/or speed. Here, we present the use of sensitive, low cost, small-sized, commercially available silicon photomultipliers (SiPMs) for the detection of radioactivity inside microfluidic channels fabricated from a range of conventional microfluidic chip substrates. We demonstrate the effects of chip material and thickness on the detection of the positron-emitting isotope, [(18)F]fluoride, and find that, while the SiPMs are light sensors, they are able to detect radiation even through opaque chip materials via direct positron and gamma (γ) ray interaction. Finally, we employed the SiPM platform for analysis of the PET (positron emission tomography) radiotracers 2-[(18)F]fluoro-2-deoxy-d-glucose ([(18)F]FDG) and [(68)Ga]gallium-citrate, and highlight the ability to detect the γ ray emitting SPECT (single photon emission computed tomography) radiotracer, [(99m)Tc]pertechnetate.
在微流控设备中检测辐射的能力对于放射性药物的芯片分析很重要，但以前报道的系统在很大程度上受到各种限制，包括成本、制造的复杂性以及灵敏度和/或速度不足。在这里，我们展示了使用灵敏、低成本、小尺寸、市售的硅光电倍增管（SiPM）来检测微流道内的放射性，这些微流道由一系列常规微流控芯片衬底制造而成。我们研究了芯片材料和厚度对正电子发射同位素[(18)F]氟化物检测的影响，发现尽管 SiPM 是光传感器，但它们能够通过直接正电子和伽马（γ）射线相互作用来检测辐射，即使是通过不透明的芯片材料。最后，我们在 SiPM 平台上分析了正电子发射断层扫描（PET）示踪剂 2-[(18)F]氟-2-脱氧-D-葡萄糖([(18)F]FDG)和[(68)Ga]镓-柠檬酸，并强调了检测发射γ射线的单光子发射计算机断层扫描（SPECT）示踪剂[(99m)Tc]高锝酸盐的能力。
