Çakir-Özkan Nilüfer, Bereket Cihan, Sener Ismail, Alici Ömer, Kabak Yonca Betil, Önger Mehmet Emin
*Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Ondokuz Mayis University, Samsun†Department of Pathology, Faculty of Medicine, Gazi Osmanpaşa University, Tokat‡Department of Pathology, Faculty of Veterinary Medicine§Department of Histology and Embryology, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey.
J Craniofac Surg. 2016 May;27(3):615-20. doi: 10.1097/SCS.0000000000002527.
No previous studies have examined the effect of sildenafil on fracture healing. This study was designed to investigate the effect of sildenafil on the fracture healing process.
Thirty-six female Sprague-Dawley rats (3-month-old) were used in this study. Animals were randomly divided into 2 groups based on treatment duration (1 week versus 4 weeks) and each group was then divided further into 2 subgroups, control (C) and study (S) groups. Group C (C1, C2) was treated daily with saline solution and group S (S1, S2) was treated daily with 10 mg/kg of sildenafil. Histologic, histomorphometric, radiological, and immunohistochemical analyses were performed at 1 week and 4 weeks after a fracture.
The sildenafil group showed a significant increase in fracture healing scores (P = 0.00). The authors observed a transition from fibrous callus to cartilage tissue and immature bone tissue in group S1; and an increased transition of cartilage tissue to completely immature bone tissue in group S2, both of which were administered sildenafil. The strong expression of bone morphogenetic protein 2 and col-1 was observed in the fibrous matrix and osteoblasts within areas of new bone formation, especially in group S1. This group also showed an increase in bone density measurements at 1 week that was statistically significant (P = 0.03).
Sildenafil accelerates fracture healing and can be used as a supporting factor in the improvement of fracture healing under various conditions.
以往尚无研究探讨西地那非对骨折愈合的影响。本研究旨在调查西地那非对骨折愈合过程的影响。
本研究使用了36只3月龄雌性斯普拉格-道利大鼠。根据治疗持续时间（1周与4周）将动物随机分为2组，然后每组再进一步分为2个亚组，即对照组（C）和研究组（S）。C组（C1、C2）每日用生理盐水治疗，S组（S1、S2）每日用10 mg/kg西地那非治疗。在骨折后1周和4周进行组织学、组织形态计量学、放射学和免疫组织化学分析。
西地那非组骨折愈合评分显著增加（P = 0.00）。作者观察到，接受西地那非治疗的S1组从纤维性骨痂过渡到软骨组织和未成熟骨组织；S2组软骨组织向完全未成熟骨组织的过渡增加。在新骨形成区域的纤维基质和成骨细胞中观察到骨形态发生蛋白2和I型胶原的强表达，尤其是在S1组。该组在1周时骨密度测量值也有增加，具有统计学意义（P = 0.03）。
西地那非可加速骨折愈合，可作为在各种情况下改善骨折愈合的辅助因素。
