Bereket C, Sener I, Cakir-Özkan N, Önger M E, Polat A V
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Ondokuz Mayis University, Samsun, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey.
Niger J Clin Pract. 2018 Feb;21(2):217-224. doi: 10.4103/njcp.njcp_172_16.
The purpose of the present study was to evaluate the effects of sildenafil on mandibular fracture healing in animals treated with zoledronic acid by using histologic, histomorphometric, immunohistochemical, and radiodensitometric methods.
A total of 36 Sprague-Dawley rats (3 months old) were used. All animals were treated intraperitoneally with 0.1 mg/kg zoledronate three times per week, for a total of 8 weeks. Postoperatively, the animals were divided into two groups: zoledronate group (Z), which had no treatment applied (n = 18), and zoledronate + sildenafil (ZS), which were treated daily with 10 mg/kg sildenafil (n = 18). Each group was divided into two subgroups and the animals were sacrificed at the end of week 1 (Z1 and ZS1, n = 9) and week 4 (Z4 and ZS4, n = 9) after the operation. Histologic, histomorphometric, immunohistochemical analysis, and radiodensitometry were performed on the test subjects.
Sildenafil-treated groups showed a significant increase in fracture healing scores. This result was supported by the densitometric, histologic, histomorphometric, and immunohistochemical findings.
Sildenafil may have positive effects on accelerating and improving fracture healing, and it may be used as a supporting factor in bone healing in patients treated with bisphosphonate (BP) to prevent negative effects of BP's.
本研究旨在通过组织学、组织形态计量学、免疫组织化学和放射密度测定法，评估西地那非对接受唑来膦酸治疗的动物下颌骨骨折愈合的影响。
总共使用了36只3个月大的Sprague-Dawley大鼠。所有动物每周接受3次腹腔注射0.1mg/kg唑来膦酸盐，共8周。术后，将动物分为两组：未接受任何治疗的唑来膦酸组（Z组，n = 18）和每天接受10mg/kg西地那非治疗的唑来膦酸+西地那非组（ZS组，n = 18）。每组再分为两个亚组，在术后第1周（Z1和ZS1，n = 9）和第4周（Z4和ZS4，n = 9）结束时处死动物。对受试对象进行组织学、组织形态计量学、免疫组织化学分析和放射密度测定。
西地那非治疗组的骨折愈合评分显著增加。这一结果得到了密度测定、组织学、组织形态计量学和免疫组织化学结果的支持。
西地那非可能对加速和改善骨折愈合有积极作用，可作为双膦酸盐（BP）治疗患者骨愈合的辅助因素，以预防BP的负面影响。
