Onger M-E, Bereket C, Sener I, Ozkan N, Senel E, Polat A-V
Department of Histology and Embryology, Faculty of Medicine, Ondokuz Mayis University, 55139 Samsun/Turkey,
Med Oral Patol Oral Cir Bucal. 2017 Mar 1;22(2):e251-e257. doi: 10.4317/medoral.21556.
In this study we examined the effects of two different repeated Extracorporeal Shock Waves (ESW) on the consolidation period of the distraction osteogenesis (DO) of the rabbit mandible using stereological, radiological and immunohistochemical methods.
DO was performed unilaterally in the mandible of 18 New Zealand rabbits (six months old, weighing between 2.5-3 kg). In the consolidation period, rabbits were divided into three groups randomly after the distraction period. The distraction zone of the mandible was received no treatment as controls (E02). Group 2 (E 5002) received ESWT (twice 500 impulses at 14 kV and 0.19 mJ/mm2 energy) in the first and fourth days of the consolidation. Group 3 (E1000*2) treated with ESWT (twice 1000 impulses at 14 kV and 0.19 mJ/mm2 energy) in the first and fourth days of the consolidation period. After the sacrification, radiologically bone mineral density, new bone formation, new fibrous tissue and new vessel formation were analyzed by stereological.
It was found a statistically significant difference between the study groups and control group in the bone mineral density measurements and the highest value was in the E10002 group. In the stereological analysis, new bone formation was highest in the E10002 group and there was a significant difference compared to the other groups (E02 and E5002) (p=0.000). The lowest connective tissue volume was found in the E5002 and there was a significant difference compared to the other groups (E02 and E10002) (p=0.000). The volume of the new vessel was highest in the E5002 and lowest in the E0*2 group. It was found statistically significant difference between the values of the study and control groups.
Interestingly, we found that repetition of the 1000 impulses ESWT accelerated the consolidation, 500 impulses ESWT extended consolidation period of the DO.
在本研究中，我们使用体视学、放射学和免疫组织化学方法，研究了两种不同的重复体外冲击波（ESW）对兔下颌骨牵张成骨（DO）巩固期的影响。
对18只新西兰兔（6个月大，体重2.5 - 3千克）的下颌骨进行单侧DO。在巩固期，牵张期结束后将兔随机分为三组。下颌骨的牵张区不做处理作为对照组（E02）。第2组（E 5002）在巩固期的第1天和第4天接受体外冲击波治疗（14 kV、0.19 mJ/mm2能量下两次500次脉冲）。第3组（E1000*2）在巩固期的第1天和第4天接受体外冲击波治疗（14 kV、0.19 mJ/mm2能量下两次1000次脉冲）。处死后，通过体视学分析放射学骨密度、新骨形成、新纤维组织和新血管形成情况。
研究组与对照组在骨密度测量方面存在统计学显著差异，最高值出现在E10002组。在体视学分析中，E10002组新骨形成最高，与其他组（E02和E5002）相比存在显著差异（p = 0.000）。E5002组结缔组织体积最低，与其他组（E02和E10002）相比存在显著差异（p = 0.000）。新血管体积在E5002组最高，在E0*2组最低。研究组和对照组的值之间存在统计学显著差异。
有趣的是，我们发现1000次脉冲的体外冲击波治疗重复使用可加速巩固，500次脉冲的体外冲击波治疗会延长DO的巩固期。
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