Muhammad T M Nabeel, Sreedevi B
Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh, India.
Vet World. 2015 Jan;8(1):103-8. doi: 10.14202/vetworld.2015.103-108. Epub 2015 Jan 29.
This study was carried out for the detection of Avibacterium paragallinarum from outbreaks of infectious coryza of poultry.
The polymerase chain reaction (PCR) was standardized for the diagnosis of infectious coryza by using infectious coryza Killed vaccine, ventri biologicals, Pune as source of DNA of A. paragallinarum. Five outbreaks of infectious coryza from Andhra Pradesh were investigated in the present study. A total of 56 infra orbital sinus swabs and 22 nasal swabs were tested by PCR.
PCR analysis showed 56 positives (71.7%) for infectious coryza out of total 78 samples tested. Of 56 infra orbital sinus swabs tested, 47 were positive (83.9%) and 9 nasal swabs (40.9%) out of 22 tested had given positive results for infectious coryza. Samples collected from birds at acute stage of disease and samples collected before treatment with antibiotics were given better results on PCR.
For preventing the economic losses associated with the disease, an early, accurate and rapid diagnosis is essential. PCR is a rapid and highly sensitive diagnostic technique which can substitute conventional cultural examination.
本研究旨在从家禽传染性鼻炎疫情中检测副鸡禽杆菌。
以浦那的传染性鼻炎灭活疫苗、ventri生物制品作为副鸡禽杆菌DNA来源，对聚合酶链反应（PCR）进行标准化，用于传染性鼻炎的诊断。本研究调查了安得拉邦的5起传染性鼻炎疫情。共对56份眶下窦拭子和22份鼻拭子进行了PCR检测。
PCR分析显示，在总共检测的78个样本中，有56个（71.7%）传染性鼻炎呈阳性。在检测的56份眶下窦拭子中，47份（83.9%）呈阳性，在检测的22份鼻拭子中有9份（40.9%）传染性鼻炎检测呈阳性。从处于疾病急性期的禽类采集的样本以及在使用抗生素治疗前采集的样本，PCR检测结果更好。
为防止与该疾病相关的经济损失，早期、准确和快速的诊断至关重要。PCR是一种快速且高度灵敏的诊断技术，可替代传统的培养检查。
