Li Yong, Li Wang, He Kai-Yu, Li Pei, Huang Yan, Nie Zhou, Yao Shou-Zhuo
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
Nanoscale. 2016 Apr 28;8(16):8591-9. doi: 10.1039/c6nr01072e. Epub 2016 Apr 6.
In natural biological systems, proteins exploit various functional peptide motifs to exert target response and activity switch, providing a functional and logic basis for complex cellular activities. Building biomimetic peptide-based bio-logic systems is highly intriguing but remains relatively unexplored due to limited logic recognition elements and complex signal outputs. In this proof-of-principle work, we attempted to address these problems by utilizing multi-functional peptide probes and the peptide-mediated nanoparticle assembly system. Here, the rationally designed peptide probes function as the dual-target responsive element specifically responsive to metal ions and enzymes as well as the mediator regulating the assembly of gold nanoparticles (AuNPs). Taking advantage of Zn2+ ions and chymotrypsin as the model inputs of metal ions and enzymes, respectively, we constructed the peptide logic system computed by the multi-functional peptide probes and outputted by the readable colour change of AuNPs. In this way, the representative binary basic logic gates (AND, OR, INHIBIT, NAND, IMPLICATION) have been achieved by delicately coding the peptide sequence, demonstrating the versatility of our logic system. Additionally, we demonstrated that the three-input combinational logic gate (INHIBIT-OR) could also be successfully integrated and applied as a multi-tasking biosensor for colorimetric detection of dual targets. This nanoparticle-based peptide logic system presents a valid strategy to illustrate peptide information processing and provides a practical platform for executing peptide computing or peptide-related multiplexing sensing, implying that the controllable nanomaterial assembly is a promising and potent methodology for the advancement of biomimetic bio-logic computation.
在天然生物系统中，蛋白质利用各种功能性肽基序来实现靶标响应和活性切换，为复杂的细胞活动提供功能和逻辑基础。构建基于仿生肽的生物逻辑系统极具吸引力，但由于逻辑识别元件有限和信号输出复杂，该领域仍相对未被探索。在这项原理验证工作中，我们试图通过利用多功能肽探针和肽介导的纳米颗粒组装系统来解决这些问题。在这里，经过合理设计的肽探针充当对金属离子和酶具有特异性响应的双靶标响应元件，同时也是调节金纳米颗粒（AuNPs）组装的介质。分别利用Zn2+离子和胰凝乳蛋白酶作为金属离子和酶的模型输入，我们构建了由多功能肽探针计算并通过AuNPs可读的颜色变化输出的肽逻辑系统。通过巧妙地编码肽序列，实现了代表性的二元基本逻辑门（与、或、非、与非、蕴含），证明了我们逻辑系统的多功能性。此外，我们证明了三输入组合逻辑门（非或）也可以成功集成并用作双靶标比色检测的多任务生物传感器。这种基于纳米颗粒的肽逻辑系统为说明肽信息处理提供了一种有效的策略，并为执行肽计算或肽相关的多重传感提供了一个实用平台，这意味着可控的纳米材料组装是推进仿生生物逻辑计算的一种有前途且有效的方法。
