Rochon D M, Tremaine J H
Agriculture Canada Research Station, Vancouver, British Columbia.
Virology. 1989 Apr;169(2):251-9. doi: 10.1016/0042-6822(89)90150-5.
The complete nucleotide sequence of the cucumber necrosis virus (CNV) genome has been determined. The genome is 4701 nucleotides in length and contains five long open reading frames (ORF). ORF1 begins at the first AUG codon at the 5' terminus and terminates at an amber codon. The predicted molecular weight of the polyprotein encoded by ORF1 is 33 kilodaltons (kDa). Readthrough of the ORF1 amber codon would yield a protein with a molecular weight of 92 kDa. Comparison of the amino acid sequence of the 92-kDa protein with the putative replicases of carnation mottle virus (CarMV) and barley yellow dwarf virus (BYDV) shows extensive sequence similarity. This suggests that the CNV 92-kDa protein is the viral replicase and, furthermore, suggests a close evolutionary relationship between CNV, CarMV, and BYDV, members of the Tombus-, Carmo-, and Luteovirus groups, respectively. Immediately following the 92-kDa protein is ORF3 which can encode a 40-kDa protein. It is identified as the coat protein based on its similarity in amino acid composition to the previously determined CNV coat protein sequence (J. H. Tremaine, 1972, Virology 48, 582-590) and on its amino acid sequence similarity with the tomato bushy stunt virus coat protein. Two nested ORFs (ORF4 and -5), in different frames, follow the coat protein gene. Although it is not known if both ORFs are expressed, they would encode proteins with predicted molecular weights of 21 and 20 kDa, respectively.
黄瓜坏死病毒（CNV）基因组的完整核苷酸序列已被确定。该基因组长度为4701个核苷酸，包含五个长开放阅读框（ORF）。ORF1从5'末端的第一个AUG密码子开始，在一个琥珀密码子处终止。由ORF1编码的多聚蛋白的预测分子量为33千道尔顿（kDa）。ORF1琥珀密码子的通读将产生一种分子量为92 kDa的蛋白质。将92 kDa蛋白质的氨基酸序列与香石竹斑驳病毒（CarMV）和大麦黄矮病毒（BYDV）的假定复制酶进行比较，发现有广泛的序列相似性。这表明CNV 92 kDa蛋白质是病毒复制酶，此外，还表明CNV、CarMV和BYDV之间存在密切的进化关系，它们分别是番茄丛矮病毒属、麝香石竹潜隐病毒属和黄症病毒属的成员。紧接在92 kDa蛋白质之后的是ORF3，它可以编码一种40 kDa的蛋白质。基于其氨基酸组成与先前确定的CNV外壳蛋白序列（J. H. Tremaine，1972，《病毒学》48，582 - 590）的相似性以及其与番茄丛矮病毒外壳蛋白的氨基酸序列相似性，它被鉴定为外壳蛋白。两个嵌套的ORF（ORF4和 - 5），处于不同的阅读框，位于外壳蛋白基因之后。虽然尚不清楚这两个ORF是否都表达，但它们将分别编码预测分子量为21 kDa和20 kDa的蛋白质。
