Ranjbar Reza, Babaie Saeed
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Electron Physician. 2016 Feb 25;8(2):1943-8. doi: 10.19082/1943. eCollection 2016 Feb.
Salmonellosis, which is caused by nontyphoid salmonella bacteria, is one of the most common foodborne diseases, and it causes gastrointestinal infections worldwide, most of which are limited gastroenteritis that requires antimicrobial treatment. The aim of this study was to investigate the effects of echinophora platyloba extract on inhibiting the growth of Salmonella typhi, Salmonella enteritidis, and salmonella choleraesuis.
Echinophora Platyloba extract was collected in the East Azarbaijan Province in Iran in June 2015. Weeds, infected plants, and dried roots were separated and removed. After drying and grinding the plant, 100 grams of powder were weighed, and the extraction of the plant was carried out by percolation. This study tested the Minimum Inhibitory Concentration (MIC) by the broth micro dilution method and Minimum Bactericidal Concentration (MBC). All of the data were analyzed by SPSS statistical software, version 22.0. One-Way ANOVA and the Duncan test were used to compare the effect of various concentrations of the extract on each type of bacteria.
Our results indicated that, in 250 mg/ml of extracts discs, the largest growth inhibition zones were formed, and they were 26.11 ± 1.16, 21.23 ± 0.89, and 19.65 ± 0.60 in S. enteritidis, S. typhi, and S. choleraesuis groups, respectively. The statistical results indicated that, in each type of bacteria, there was a statistical difference (p < 0.01) between the various concentrations of the extracts and the chloramphenicol discs. Also, it was indicated that this extract at a concentration of 150 mg/ml had a germicidal effect on S. enteritidis and S. typhi bacteria and that 250 mg/ml had a bactericidal effect on S. choleraesuis.
The results of this study indicated that E. platyloba extract has potential effects as antimicrobial agents.
由非伤寒沙门氏菌引起的沙门氏菌病是最常见的食源性疾病之一，它在全球范围内导致胃肠道感染，其中大多数是需要抗菌治疗的局限性肠胃炎。本研究的目的是调查锥叶棘豆提取物对伤寒沙门氏菌、肠炎沙门氏菌和猪霍乱沙门氏菌生长的抑制作用。
2015年6月在伊朗东阿塞拜疆省采集锥叶棘豆提取物。分离并去除杂草、受感染的植物和干燥的根。将植物干燥并研磨后，称取100克粉末，采用渗漉法进行植物提取。本研究通过肉汤微量稀释法检测最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。所有数据均使用SPSS 22.0统计软件进行分析。采用单因素方差分析和邓肯检验比较不同浓度提取物对每种细菌的作用效果。
我们的结果表明，在250毫克/毫升提取物圆片中，形成的最大生长抑制圈分别在肠炎沙门氏菌、伤寒沙门氏菌和猪霍乱沙门氏菌组中，为26.11±1.16、21.23±0.89和19.65±0.60。统计结果表明，在每种细菌中，不同浓度提取物与氯霉素圆片之间存在统计学差异（p<0.01）。此外，结果表明该提取物在150毫克/毫升浓度下对肠炎沙门氏菌和伤寒沙门氏菌有杀菌作用，在250毫克/毫升浓度下对猪霍乱沙门氏菌有杀菌作用。
本研究结果表明锥叶棘豆提取物具有作为抗菌剂的潜在作用。
