Young Researcher Club, Karaj Branch, Islamic Azad University, Karaj, Iran.
Jpn J Infect Dis. 2010 Nov;63(6):417-21.
Salmonella enterica has become progressively resistant to antimicrobial agents worldwide as a result of genes carried on different classes of integrons. The aim of the current study was to investigate the molecular diversity of these integrons and their association with antimicrobial resistance in clinical S. enterica isolates from Tehran, Iran. Antimicrobial susceptibility testing was performed according to the Clinical and Laboratory Standards Institute. The presence of integrons was investigated by PCR using specific primers. Integrons were detected in 65 (47.1%) strains, with classes 1 and 2 being observed in 54 (39%) and 11 (8%) strains, respectively. Integron-positive isolates belonged to seven different S. enterica serovars, and all showed a multidrug-resistant (MDR) phenotype. Our findings show that integrons are widely disseminated among S. enterica strains from Tehran. Furthermore, the results that class 1 integrons were more prevalent than class 2 in Salmonella isolates, and that a statistical association with MDR patterns was observed, suggest that they are more likely to be important in conferring a resistant phenotype to Salmonella strains.
由于不同类别整合子上携带的基因，沙门氏菌已在全球范围内对抗菌药物产生了越来越强的耐药性。本研究旨在调查这些整合子的分子多样性及其与来自伊朗德黑兰的临床沙门氏菌分离株的抗菌药物耐药性的关系。根据临床和实验室标准协会进行抗菌药物敏感性测试。使用特异性引物通过 PCR 检测整合子的存在。在 65 株（47.1%）菌株中检测到整合子，其中 54 株（39%）和 11 株（8%）菌株分别观察到 1 类和 2 类整合子。整合子阳性分离株属于 7 种不同的沙门氏菌血清型，均表现出多药耐药（MDR）表型。我们的研究结果表明，整合子在来自德黑兰的沙门氏菌菌株中广泛传播。此外，结果表明，沙门氏菌分离株中 1 类整合子比 2 类更为普遍，并且观察到与 MDR 模式存在统计学关联，这表明它们更有可能在赋予沙门氏菌菌株耐药表型方面发挥重要作用。
