Vongpaisarnsin Kornkiat, Boonlert Achara, Rasmeepaisarn Kawin, Dangkao Piyawan
Department of Forensic Medicine, Faculty of Medicine, Chulalongkorn University, 1873 Rama4 road, Lumpini, Bangkok, 10330, Thailand.
Forensic Serology and DNA, King Chulalongkorn Memorial Hospital, 1873 Rama4 road, Lumpini, Bangkok, 10330, Thailand.
Int J Legal Med. 2016 Nov;130(6):1497-1499. doi: 10.1007/s00414-016-1363-y. Epub 2016 Apr 8.
Genetic data from 12 short tandem repeats (STR) on the X chromosome are currently used in forensics studies to resolve issues related to complex kinship or when data is missing or ambiguous. In this study, we genotyped these 12 X chromosome STR in DNA collected from individuals from central Thailand (n = 391, 282 men and 109 women) and used this information to calculate allele and haplotype frequencies as well as forensic parameters for kinship calculations. Polymorphism information contents of the loci were range from 0.5283-0.9247, and powers of discrimination in females and males were 0.7666-0.9905 and 0.6085-0.9291, respectively. A diallelic pattern was observed at the locus DXS7132. Moreover, a comparison of genetic distance revealed a close relationship within Asian countries. Our results indicate that the X chromosomal short tandem repeat (X-STR) multiplex system provides highly informative genetic data and could be advantageous in forensic studies.
目前，来自X染色体上12个短串联重复序列（STR）的遗传数据被用于法医学研究，以解决与复杂亲属关系相关的问题，或在数据缺失或模糊不清时使用。在本研究中，我们对从泰国中部个体（n = 391，282名男性和109名女性）收集的DNA中的这12个X染色体STR进行了基因分型，并利用这些信息计算等位基因和单倍型频率以及亲属关系计算的法医学参数。这些位点的多态性信息含量范围为0.5283 - 0.9247，女性和男性的鉴别力分别为0.7666 - 0.9905和0.6085 - 0.9291。在DXS7132位点观察到双等位基因模式。此外，遗传距离的比较揭示了亚洲国家之间的密切关系。我们的结果表明，X染色体短串联重复序列（X-STR）多重系统提供了高度信息丰富的遗传数据，在法医学研究中可能具有优势。
