Abreu Murilo S, Giacomini Ana C V V, Koakoski Gessi, Piato Angelo L, Barcellos Leonardo J G
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Av. Roraima, 1000, Cidade Universitária, Camobi, Santa Maria, RS 97105-900, Brazil.
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Av. Roraima, 1000, Cidade Universitária, Camobi, Santa Maria, RS 97105-900, Brazil; Universidade de Passo Fundo (UPF), BR 285, São José, Passo Fundo, RS 99052-900, Brazil.
Physiol Behav. 2016 Jun 1;160:59-65. doi: 10.1016/j.physbeh.2016.04.003. Epub 2016 Apr 8.
Jundiá (Rhamdia quelen) is a suitable species for aquaculture in regions of temperate or subtropical climate. This species has received great attention regarding several aspects of physiology as well as an organism to study the impact of environmental contaminations. However, experiments using validated and objective tests to evaluate the jundiá behavior are scarce. The effects of acute stress have been studied in other fish species, such as zebrafish (Danio rerio), however, the effects in jundiá are lacking. Thus, we evaluated the effects of acute stress (net chasing) on anxiety-like and social behavior in jundiá. For these purpose, all behavioral analyses were carried out using automated tracking software. We showed that the acute stress protocol increased cortisol levels and induced anxiogenic-like behavior in the novel tank test, and decreased social behavior in jundiá. The antidepressant fluoxetine was able to prevent the effects of acute stress on social behavior. Here we show a behavioral evaluation of Rhamdia quelen using consolidated tests and computerized analysis, which allows more measurable, reliable and comparable results.
军曹鱼（Rhamdia quelen）是温带或亚热带气候地区适合水产养殖的物种。该物种在生理学的多个方面以及作为研究环境污染影响的生物体方面受到了极大关注。然而，使用经过验证的客观测试来评估军曹鱼行为的实验却很少。急性应激的影响已在其他鱼类物种中进行了研究，例如斑马鱼（Danio rerio），但军曹鱼的相关影响尚不清楚。因此，我们评估了急性应激（网捕追逐）对军曹鱼焦虑样行为和社交行为的影响。为此，所有行为分析均使用自动跟踪软件进行。我们发现，急性应激方案会增加皮质醇水平，并在新水箱测试中诱发焦虑样行为，同时还会降低军曹鱼的社交行为。抗抑郁药氟西汀能够预防急性应激对社交行为的影响。在此，我们展示了使用综合测试和计算机分析对军曹鱼进行的行为评估，这使得结果更具可测量性、可靠性和可比性。
