Nakamura A, Nakashima M, Sakai K, Niwa M, Nozaki M, Shiomi H
Department of Pharmacology, Faculty of Pharmacy, Fukuyama University, Japan.
Brain Res. 1989 Feb 27;481(1):165-8. doi: 10.1016/0006-8993(89)90498-8.
We studied whether delta-sleep-inducing peptide (DSIP) acted on opioid receptor directly or indirectly. DSIP did not have binding activity to any subtype of opioid receptors. DSIP at doses of 1 pM-1 nM significantly stimulated the release of immunoreactive Met-enkephalin (iME) from superfused slices of the rat lower brainstem. The DSIP-induced release of iME was calcium-dependent. These results show that DSIP acts on opioid receptor indirectly by stimulating the release of iME in producing antinociceptive effects.
我们研究了诱导δ波睡眠肽（DSIP）是直接还是间接作用于阿片受体。DSIP对任何亚型的阿片受体均无结合活性。剂量为1皮摩尔至1纳摩尔的DSIP能显著刺激大鼠低位脑干灌流切片中免疫反应性甲硫氨酸脑啡肽（iME）的释放。DSIP诱导的iME释放是钙依赖性的。这些结果表明，DSIP在产生抗伤害感受作用时，通过刺激iME的释放间接作用于阿片受体。
