Kucki M, Rupper P, Sarrieu C, Melucci M, Treossi E, Schwarz A, León V, Kraegeloh A, Flahaut E, Vázquez E, Palermo V, Wick P
Laboratory for Particles-Biology Interactions, Swiss Federal Laboratories for Materials Science and Technology (Empa), Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland.
Nanoscale. 2016 Apr 28;8(16):8749-60. doi: 10.1039/c6nr00319b. Epub 2016 Apr 11.
Graphene-related materials (GRM) inherit unique combinations of physicochemical properties which offer a high potential for technological as well as biomedical applications. It is not clear which physicochemical properties are the most relevant factors influencing the behavior of GRM in complex biological environments. In this study we have focused on the interaction of GRM, especially graphene oxide (GO), and Caco-2 cells in vitro. We mimiked stomach transition by acid-treatment of two representative GRM followed by analysis of their physicochemical properties. No significant changes in the material properties or cell viability of exposed Caco-2 cells in respect to untreated GRM could be detected. Furthermore, we explored the interaction of four different GO and Caco-2 cells to identify relevant physicochemical properties for the establishment of a material property-biological response relationship. Despite close interaction with the cell surface and the formation of reactive oxygen species (ROS), no acute toxicity was found for any of the applied GO (concentration range 0-80 μg ml(-1)) after 24 h and 48 h exposure. Graphene nanoplatelet aggregates led to low acute toxicity at high concentrations, indicating that aggregation, the number of layers or the C/O ratio have a more pronounced effect on the cell viability than the lateral size alone.
石墨烯相关材料（GRM）具有独特的物理化学性质组合，在技术和生物医学应用方面具有很高的潜力。目前尚不清楚哪些物理化学性质是影响GRM在复杂生物环境中行为的最相关因素。在本研究中，我们重点关注了GRM，特别是氧化石墨烯（GO）与Caco-2细胞的体外相互作用。我们通过对两种代表性GRM进行酸处理来模拟胃部转变，然后分析它们的物理化学性质。与未处理的GRM相比，未检测到暴露的Caco-2细胞的材料性质或细胞活力有显著变化。此外，我们研究了四种不同的GO与Caco-2细胞的相互作用，以确定建立材料性质-生物反应关系的相关物理化学性质。尽管与细胞表面有密切相互作用并形成了活性氧（ROS），但在暴露24小时和48小时后，所应用的任何GO（浓度范围为0-80μg ml(-1)）均未发现急性毒性。石墨烯纳米片聚集体在高浓度下导致低急性毒性，这表明聚集、层数或C/O比单独对细胞活力的影响比横向尺寸更显著。
