Singh A, Singh A, Chaudhri S S
Immunology Section, Department of Veterinary Microbiology, Ch. Charan Singh Haryana Agricultural University, Hisar (Haryana), India.
Parasite Immunol. 2004 Apr;26(4):167-75. doi: 10.1111/j.0141-9838.2004.00697.x.
Polypeptide profiles of Schistosoma indicum and S. spindale adult worm homogenates were obtained by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Humoral immune status of infected cattle, sheep and goats against Schistosoma indicum and S. spindale Ags was determined by immunoblot analysis and by indirect ELISA using four major polypeptides of approximate molecular masses 45 kDa, 40 kDa, 28 kDa and 15 kDa electro-eluted from the gel slices. Cattle sera samples had higher levels of antibodies against Si/s40 and Si/s28 than against Si/s45 antigen. Reasons have been discussed for the absence of detectable levels of anti-Si/s28, -Si/s45 and -Si/s40 antibodies in a significant number of sera samples from S. indicum egg-positive sheep.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳获得了印度血吸虫和梭形血吸虫成虫匀浆的多肽图谱。采用免疫印迹分析以及间接ELISA方法，利用从凝胶切片上电洗脱得到的四种近似分子量分别为45 kDa、40 kDa、28 kDa和15 kDa的主要多肽，测定了感染牛、羊和山羊针对印度血吸虫和梭形血吸虫抗原的体液免疫状态。牛血清样本中针对Si/s40和Si/s28的抗体水平高于针对Si/s45抗原的抗体水平。对于大量来自印度血吸虫虫卵阳性绵羊的血清样本中未检测到抗Si/s28、抗Si/s45和抗Si/s40抗体的原因进行了讨论。
