Nagel Manuela, Kodde Jan, Pistrick Sibylle, Mascher Martin, Börner Andreas, Groot Steven P C
Genebank Department, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK Gatersleben) Stadt Seeland, Germany.
Wageningen UR, Plant Research International B.V. Wageningen, Netherlands.
Front Plant Sci. 2016 Mar 31;7:388. doi: 10.3389/fpls.2016.00388. eCollection 2016.
Experimental seed aging approaches intend to mimic seed deterioration processes to achieve a storage interval reduction. Common methods apply higher seed moisture levels and temperatures. In contrast, the "elevated partial pressure of oxygen" (EPPO) approach treats dry seed stored at ambient temperatures with high oxygen pressure. To analyse the genetic background of seed longevity and the effects of seed aging under dry conditions, the EPPO approach was applied to the progeny of the Oregon Wolfe Barley (OWB) mapping population. In comparison to a non-treated control and a control high-pressure nitrogen treatment, EPPO stored seeds showed typical symptoms of aging with a significant reduction of normal seedlings, slower germination, and less total germination. Thereby, the parent Dom ("OWB-D"), carrying dominant alleles, is more sensitive to aging in comparison to the population mean and in most cases to the parent Rec ("OWB-R"), carrying recessive alleles. Quantitative trait locus (QTL) analyses using 2832 markers revealed 65 QTLs, including two major loci for seed vigor on 2H and 7H. QTLs for EPPO tolerance were detected on 3H, 4H, and 5H. An applied controlled deterioration (CD) treatment (aged at higher moisture level and temperature) revealed a tolerance QTL on 5H, indicating that the mechanism of seed deterioration differs in part between EPPO or CD conditions.
实验性种子老化方法旨在模拟种子劣变过程，以缩短储存间隔。常用方法是采用较高的种子水分含量和温度。相比之下，“高氧分压”（EPPO）方法是在环境温度下用高氧压处理干燥种子。为了分析种子寿命的遗传背景以及干燥条件下种子老化的影响，EPPO方法应用于俄勒冈沃尔夫大麦（OWB）作图群体的后代。与未处理的对照和高压氮气处理的对照相比，经EPPO处理储存的种子表现出典型的老化症状，正常幼苗显著减少，发芽速度减慢，总发芽率降低。因此，携带显性等位基因的亲本Dom（“OWB-D”）与群体平均值相比，在大多数情况下与携带隐性等位基因的亲本Rec（“OWB-R”）相比，对老化更敏感。使用2832个标记进行的数量性状位点（QTL）分析揭示了65个QTL，包括位于2H和7H上的两个种子活力主要位点。在3H、4H和5H上检测到了对EPPO耐受性的QTL。应用的控制劣变（CD）处理（在较高水分含量和温度下老化）在5H上揭示了一个耐受性QTL，表明在EPPO或CD条件下，种子劣变机制部分不同。
