Zuo Jinghong, Liu Jindong, Gao Fengmei, Yin Guihong, Wang Zhi, Chen Fengying, Li Xiaoying, Xu Jimei, Chen Tiantian, Li Lei, Li Yu, Xia Xianchun, Cao Hong, Liu Yongxiu
Key Laboratory of Plant Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing, China.
College of Life Science, University of Chinese Academy of Sciences, Beijing, China.
Front Plant Sci. 2018 Jul 27;9:1101. doi: 10.3389/fpls.2018.01101. eCollection 2018.
Long-term storage of seeds leads to lose seed vigor with slow and non-uniform germination. Time, rate, homogeneity, and synchrony are important aspects during the dynamic germination process to assess seed viability after storage. The aim of this study is to identify quantitative trait loci (QTLs) using a high-density genetic linkage map of common wheat () for seed vigor-related traits under artificial aging. Two hundred and forty-six recombinant inbred lines derived from the cross between Zhou 8425B and Chinese Spring were evaluated for seed storability. Ninety-six QTLs were detected on all wheat chromosomes except 2B, 4D, 6D, and 7D, explaining 2.9-19.4% of the phenotypic variance. These QTLs were clustered into 17 QTL-rich regions on chromosomes 1AL, 2DS, 3AS (3), 3BS, 3BL (2), 3DL, 4AS, 4AL (3), 5AS, 5DS, 6BL, and 7AL, exhibiting pleiotropic effects. Moreover, 10 stable QTLs were identified on chromosomes 2D, 3D, 4A, and 6B (, , , , , , , , , and ). Our results indicate that one of the stable QTL-rich regions on chromosome 2D flanked by and in the position from 46 to 51 cM, presenting as a pleiotropic locus strongly impacting seed vigor-related traits under artificial aging. These new QTLs and tightly linked SNP markers may provide new valuable information and could serve as targets for fine mapping or markers assisted breeding.
种子的长期储存会导致种子活力丧失，发芽缓慢且不均匀。在动态发芽过程中，时间、速率、均匀性和同步性是评估储存后种子活力的重要方面。本研究的目的是利用普通小麦（）的高密度遗传连锁图谱，鉴定与人工老化条件下种子活力相关性状的数量性状位点（QTL）。对由周8425B和中国春杂交产生的246个重组自交系进行种子耐储性评估。在除2B、4D、6D和7D之外的所有小麦染色体上检测到96个QTL，解释了表型变异的2.9 - 19.4%。这些QTL聚集在1AL、2DS、3AS（3个）、3BS、3BL（2个）、3DL、4AS、4AL（3个）、5AS、5DS、6BL和7AL染色体上的17个QTL富集区域，表现出多效性效应。此外，在2D、3D、4A和6B染色体上鉴定出10个稳定的QTL（、、、、、、、、、和）。我们的结果表明，2D染色体上一个稳定的QTL富集区域位于和之间，位置在46至51 cM处，表现为一个对人工老化条件下种子活力相关性状有强烈影响的多效性位点。这些新的QTL和紧密连锁的SNP标记可能提供新的有价值信息，并可作为精细定位或标记辅助育种的目标。
