Wallace Sean W, Singhvi Aakanksha, Liang Yupu, Lu Yun, Shaham Shai
Laboratory of Developmental Genetics, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
CCTS Research Bioinformatics, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
Cell Rep. 2016 Apr 19;15(3):550-562. doi: 10.1016/j.celrep.2016.03.051. Epub 2016 Apr 7.
Sensory neurons are an animal's gateway to the world, and their receptive endings, the sites of sensory signal transduction, are often associated with glia. Although glia are known to promote sensory-neuron functions, the molecular bases of these interactions are poorly explored. Here, we describe a post-developmental glial role for the PROS-1/Prospero/PROX1 homeodomain protein in sensory-neuron function in C. elegans. Using glia expression profiling, we demonstrate that, unlike previously characterized cell fate roles, PROS-1 functions post-embryonically to control sense-organ glia-specific secretome expression. PROS-1 functions cell autonomously to regulate glial secretion and membrane structure, and non-cell autonomously to control the shape and function of the receptive endings of sensory neurons. Known glial genes controlling sensory-neuron function are PROS-1 targets, and we identify additional PROS-1-dependent genes required for neuron attributes. Drosophila Prospero and vertebrate PROX1 are expressed in post-mitotic sense-organ glia and astrocytes, suggesting conserved roles for this class of transcription factors.
感觉神经元是动物与外界联系的通道，其感受末梢作为感觉信号转导的位点，常与神经胶质细胞相关联。虽然已知神经胶质细胞能促进感觉神经元的功能，但这些相互作用的分子基础却鲜有研究。在此，我们描述了线虫中PROS - 1/Prospero/PROX1同源结构域蛋白在感觉神经元功能中发育后的神经胶质细胞作用。通过神经胶质细胞表达谱分析，我们证明，与之前所描述的细胞命运作用不同，PROS - 1在胚胎后期发挥作用，以控制感觉器官神经胶质细胞特异性分泌组的表达。PROS - 1在细胞自主水平上调节神经胶质细胞的分泌和膜结构，在非细胞自主水平上控制感觉神经元感受末梢的形状和功能。已知控制感觉神经元功能的神经胶质细胞基因是PROS - 1的靶标，我们还鉴定出了神经元特性所需的其他PROS - 1依赖性基因。果蝇的Prospero和脊椎动物的PROX1在有丝分裂后的感觉器官神经胶质细胞和星形胶质细胞中表达，这表明这类转录因子具有保守作用。
