Shah Asma Tufail, Batool Madeeha, Chaudhry Aqif Anwar, Iqbal Farasat, Javaid Ayesha, Zahid Saba, Ilyas Kanwal, Bin Qasim Saad, Khan Ather Farooq, Khan Abdul Samad, Ur Rehman Ihtesham
Interdisciplinary Research Centre in Biomedical Materials, COMSATS Institute of Information Technology, Lahore 54600, Pakistan.
Institute of Chemistry, University of the Punjab, Lahore 54000, Pakistan.
J Mech Behav Biomed Mater. 2016 Aug;61:617-626. doi: 10.1016/j.jmbbm.2016.03.030. Epub 2016 Apr 6.
In this manuscript for the first time calcium hydroxide (Ca(OH)2) has been used for preparation of bioactive glass (BG-2) by co-precipitation method and compared with glass prepared using calcium nitrate tetrahydrate Ca(NO3)2·4H2O (BG-1), which is a conventional source of calcium. The new source positively affected physical, biological and mechanical properties of BG-2. The glasses were characterized by Fourier transform infrared (FTIR), X-Ray Diffractometer (XRD), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis/Differential Scanning Calorimetry (TGA-DSC), BET surface area analysis and Knoop hardness. The results showed that BG-2 possessed relatively larger surface properties (100m(2)g(-1) surface area) as compared to BG-1 (78m(2)g(-1)), spherical morphology and crystalline phases (wollastonite and apatite) after sintering at lower than conventional temperature. These properties contribute critical role in both mechanical and biological properties of glasses. The Knoop hardness measurements revealed that BG-2 possessed much better hardness (0.43±0.06GPa at 680°C and 2.16±0.46GPa at 980°C) than BG-1 (0.24±0.01 at 680°C and 0.57±0.07GPA at 980°C) under same conditions. Alamar blue Assay and confocal microscopy revealed that BG-2 exhibited better attachment and proliferation of MG63 cells. Based on the improved biological properties of BG-2 as a consequent of novel calcium source selection, BG-2 is proposed as a bioactive ceramic for hard tissue repair and regeneration applications.
在本手稿中，首次使用氢氧化钙（Ca(OH)₂）通过共沉淀法制备生物活性玻璃（BG - 2），并与使用四水合硝酸钙Ca(NO₃)₂·4H₂O制备的玻璃（BG - 1）进行比较，Ca(NO₃)₂·4H₂O是钙的传统来源。这种新来源对BG - 2的物理、生物学和机械性能产生了积极影响。通过傅里叶变换红外光谱（FTIR）、X射线衍射仪（XRD）、扫描电子显微镜（SEM）、热重分析/差示扫描量热法（TGA - DSC）、BET比表面积分析和努氏硬度对玻璃进行了表征。结果表明，与BG - 1（78m²g⁻¹）相比，BG - 2具有相对更大的表面性能（100m²g⁻¹比表面积），在低于传统温度下烧结后具有球形形态和晶相（硅灰石和磷灰石）。这些性能在玻璃的机械和生物学性能中都起着关键作用。努氏硬度测量表明，在相同条件下，BG - 2的硬度（680°C时为0.43±0.06GPa，980°C时为2.16±0.46GPa）比BG - 1（680°C时为0.24±0.01，980°C时为0.57±0.07GPA）好得多。阿拉玛蓝检测和共聚焦显微镜显示，BG - 2表现出更好的MG63细胞附着和增殖。基于新型钙源选择导致BG - 2生物学性能的改善，BG - 2被提议作为一种用于硬组织修复和再生应用的生物活性陶瓷。
