Fekrazad Reza, Asefi Sohrab, Allahdadi Mahdi, Kalhori Katayoun A M
1 Dental Faculty, Periodontology Department, AJA University of Medical sciences , Tehran, Iran .
2 Postgraduate of Orthodontics, Shahid Beheshti University of Medical Sciences , Tehran, Iran .
Photomed Laser Surg. 2016 Nov;34(11):533-542. doi: 10.1089/pho.2015.4029. Epub 2016 Apr 12.
The purpose of this study was to review available literature about the effect of photobiomodulation (PBM) on mesenchymal stem cells (MSCs).
The effects of coherent and noncoherent light sources such as low-level lasers and light-emitting diodes (LEDs) on cells and tissues, known as PBM, form the basis of photomedicine. This treatment technique effects cell function, proliferation, and migration, and plays an important role in tissue regeneration. Stem cells have been found to be helpful elements in tissue regeneration, and the combination of stem cell therapy and laser therapy appears to positively affect treatment results.
An electronic search in PubMed was conducted of publications from the previous 12 years. English language articles related to the subject were found using selected key words. The full texts of potentially suitable articles were assessed according to inclusion and exclusion criteria.
After evaluation, 30 articles were deemed relevant according to the inclusion criteria. The energy density of the laser was 0.7-9 J/cm. The power used for visible light was 30-110 mW and that used for infrared light was 50-800 mW. Nearly all studies showed that low-level laser therapy had a positive effect on cell proliferation. Similar outcomes were found for LED; however, some studies suggest that the laser alone is not effective, and should be used as an adjunct tool.
PBM has positive effects on MSCs. This review concluded that doses of 0.7-4 J/cm and wavelengths of 600-700 nm are appropriate for light therapy. The results were dependent upon different parameters; therefore, optimization of parameters used in light therapy to obtain favorable results is required to provide more accurate comparison.
本研究旨在回顾关于光生物调节（PBM）对间充质干细胞（MSCs）影响的现有文献。
诸如低强度激光和发光二极管（LED）等相干和非相干光源对细胞和组织的影响，即PBM，构成了光医学的基础。这种治疗技术影响细胞功能、增殖和迁移，并在组织再生中发挥重要作用。干细胞已被发现是组织再生中的有益元素，干细胞疗法与激光疗法的结合似乎对治疗结果有积极影响。
在PubMed中对过去12年的出版物进行电子检索。使用选定的关键词查找与该主题相关的英文文章。根据纳入和排除标准评估潜在合适文章的全文。
评估后，根据纳入标准有30篇文章被认为相关。激光的能量密度为0.7 - 9 J/cm²。用于可见光的功率为30 - 110 mW，用于红外光的功率为50 - 800 mW。几乎所有研究都表明低强度激光疗法对细胞增殖有积极影响。LED也有类似结果；然而，一些研究表明单独使用激光无效，应作为辅助工具使用。
PBM对MSCs有积极影响。本综述得出结论，0.7 - 4 J/cm²的剂量和600 - 700 nm的波长适用于光疗。结果取决于不同参数；因此，需要优化光疗中使用的参数以获得良好结果，以便进行更准确的比较。
