Department of Health Sciences, Postgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto Medical School, University of São Paulo, Bandeirantes Avenue, 3900, Monte Alegre, CEP 14049-900, Ribeirão Preto, São Paulo, Brazil.
Cell Biology Laboratory, Ribeirão Preto Blood Center Foundation, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Lasers Med Sci. 2024 Aug 1;39(1):205. doi: 10.1007/s10103-024-04159-z.
Mesenchymal stem cells can differentiate into specific cell lineages in the tissue repair process. Photobiomodulation with laser and LED is used to treat several comorbidities, can interfere in cell proliferation and viability, in addition to promoting responses related to the physical parameters adopted. Evaluate and compare the effects of laser and LED on mesenchymal cells, with different energy doses and different wavelengths, in addition to viability and wound closure. Mesenchymal stem cells derived from human adipocytes were irradiated with laser (energy of 0.5 J, 2 J and 4 J, wavelength of 660 nm and 830 nm), and LED (energy of 0.5 J, 2 J and 4 J, where lengths are 630 nm and 850 nm). The wound closure process was evaluated through monitoring the reduction of the lesion area in vitro. Viability was determined by analysis with Hoechst and Propidium Iodide markers, and quantification of viable and non-viable cells respectively Data distributions were analyzed using the Shapiro-Wilk test. Homogeneity was analyzed using Levene's test. The comparison between the parameters used was analyzed using the Two-way ANOVA test. The T test was applied to data relating to viability and lesion area. For LED photobiomodulation, only the 630 nm wavelength obtained a significant result in 24, 48 and 72 h (p = 0,027; p = 0,024; p = 0,009). The results related to the in vitro wound closure test indicate that both photobiomodulation with laser and LED demonstrated significant results considering the time it takes to approach the edges (p < 0.05). Considering the in vitro experimental conditions of the study, it is possible to conclude that the physical parameters of photobiomodulation, such as energy and wavelength, with laser or LED in mesenchymal stem cells, can play a potential role in cell viability and wound closure.
间充质干细胞可以在组织修复过程中分化为特定的细胞谱系。光生物调节使用激光和 LED 来治疗多种合并症，可以干扰细胞增殖和活力，除了促进与所采用的物理参数相关的反应。评估和比较激光和 LED 对间充质细胞的影响，使用不同的能量剂量和不同的波长，以及活力和伤口闭合。从人脂肪细胞中分离的间充质干细胞用激光（能量为 0.5 J、2 J 和 4 J，波长为 660 nm 和 830 nm）和 LED（能量为 0.5 J、2 J 和 4 J，波长为 630 nm 和 850 nm）照射。通过监测体外病变面积的减少来评估伤口闭合过程。活力通过用 Hoechst 和碘化丙啶标记物进行分析来确定，并分别定量计算活细胞和非活细胞。数据分布使用 Shapiro-Wilk 检验进行分析。使用 Levene 检验分析同质性。使用双向方差分析检验分析参数之间的比较。T 检验应用于与活力和病变面积相关的数据。对于 LED 光生物调节，只有 630nm 波长在 24、48 和 72 小时时获得显著结果（p=0.027;p=0.024;p=0.009）。与体外伤口闭合试验相关的结果表明，激光和 LED 的光生物调节都在接近边缘的时间上表现出显著结果（p<0.05）。考虑到研究的体外实验条件，可以得出结论，光生物调节的物理参数，如能量和波长，使用激光或 LED 可以在间充质干细胞中发挥潜在的作用，包括细胞活力和伤口闭合。
