Natural Products Center, School of Natural Resources and the Environment, College of Agriculture and Life Sciences, University of Arizona , 250 E. Valencia Road, Tucson, Arizona 85706, United States.
J Nat Prod. 2016 Apr 22;79(4):821-30. doi: 10.1021/acs.jnatprod.5b00911. Epub 2016 Apr 12.
When cultivated under aeroponic growth conditions, Physalis crassifolia produced 11 new withanolides (1-11) and seven known withanolides (12-18) including those obtained from the wild-crafted plant. The structures of the new withanolides were elucidated by the application of spectroscopic techniques, and the known withanolides were identified by comparison of their spectroscopic data with those reported. Withanolides 1-11 and 16 were evaluated for their potential anticancer activity using five tumor cell lines. Of these, the 17β-hydroxy-18-acetoxywithanolides 1, 2, 6, 7, and 16 showed potent antiproliferative activity, with some having selectivity for prostate adenocarcinoma (LNCaP and PC-3M) compared to the breast adenocarcinoma (MCF-7), non-small-cell lung cancer (NCI-H460), and CNS glioma (SF-268) cell lines used. The cytotoxicity data obtained for 12-15, 17, and 19 have provided additional structure-activity relationship information for the 17β-hydroxy-18-acetoxywithanolides.
在气培生长条件下培养时，酸浆产生了 11 种新的羽扇豆烷类化合物（1-11）和 7 种已知的羽扇豆烷类化合物（12-18），包括从野生植物中获得的化合物。通过应用光谱技术阐明了新羽扇豆烷类化合物的结构，通过比较其光谱数据与已报道的数据，鉴定了已知的羽扇豆烷类化合物。使用五种肿瘤细胞系评估了化合物 1-11 和 16 的潜在抗癌活性。在这些化合物中，17β-羟基-18-乙酰氧基羽扇豆烷类化合物 1、2、6、7 和 16 表现出很强的抗增殖活性，与用于测试的乳腺癌（MCF-7）、非小细胞肺癌（NCI-H460）和中枢神经系统神经胶质瘤（SF-268）细胞系相比，对前列腺腺癌（LNCaP 和 PC-3M）具有选择性。对于 12-15、17 和 19 的细胞毒性数据提供了 17β-羟基-18-乙酰氧基羽扇豆烷类化合物的更多结构-活性关系信息。
