Natural Products Center, School of Natural Resources and the Environment, College of Agriculture and Life Sciences, The University of Arizona, 250 E. Valencia Road, Tucson, AZ 85706, United States.
Basic Research Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, National Cancer Institute-Frederick, Maryland 21702, United States; Cancer and Inflammation Program, National Cancer Institute-Frederick, Maryland 21702, United States.
Phytochemistry. 2018 Aug;152:174-181. doi: 10.1016/j.phytochem.2018.04.018. Epub 2018 May 21.
Eleven withanolides including six previously undescribed compounds, 16β-hydroxyixocarpanolide, 24,25-dihydroexodeconolide C, 16,17-dehydro-24-epi-dioscorolide A, 17-epi-philadelphicalactone A, 16-deoxyphiladelphicalactone C, and 4-deoxyixocarpalactone A were isolated from aeroponically grown Physalis philadelphica. Structures of these withanolides were elucidated by the analysis of their spectroscopic (HRMS, 1D and 2D NMR, ECD) data and comparison with published data for related withanolides. Cytotoxic activity of all isolated compounds was evaluated against a panel of five human tumor cell lines (LNCaP, ACHN, UO-31, M14 and SK-MEL-28), and normal (HFF) cells. Of these, 17-epi-philadelphicalactone A, withaphysacarpin, philadelphicalactone C, and ixocarpalactone A exhibited cytotoxicity against ACHN, UO-31, M14 and SK-MEL-28, but showed no toxicity to HFF cells.
从气培的酸浆中分离得到了 11 个醉茄内酯，包括 6 个以前未描述的化合物：16β-羟基 ixocarpanolide、24,25-二氢 exodeconolide C、16,17-脱氢-24-表 dioscorolide A、17-表 philadelphicalactone A、16-脱氧 philadelphicalactone C 和 4-脱氧 ixocarpalactone A。通过分析这些醉茄内酯的光谱（高分辨质谱、1D 和 2D NMR、ECD）数据，并与相关醉茄内酯的已发表数据进行比较，确定了这些醉茄内酯的结构。对所有分离得到的化合物进行了对一组 5 个人类肿瘤细胞系（LNCaP、ACHN、UO-31、M14 和 SK-MEL-28）和正常（HFF）细胞的细胞毒性评估。其中，17-表 philadelphicalactone A、withaphysacarpin、philadelphicalactone C 和 ixocarpalactone A 对 ACHN、UO-31、M14 和 SK-MEL-28 具有细胞毒性，但对 HFF 细胞没有毒性。
