通过敲除6-磷酸果糖激酶I并阻断大肠杆菌中的Entner-Doudoroff途径提高核黄素产量。
Increased riboflavin production by knockout of 6-phosphofructokinase I and blocking the Entner-Doudoroff pathway in Escherichia coli.
作者信息
Liu Shuang, Kang Pei, Cui Zhenzhen, Wang Zhiwen, Chen Tao
机构信息
Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.
Key Laboratory of System Bioengineering, Ministry of Education, Tianjin University, Tianjin, 300072, People's Republic of China.
出版信息
Biotechnol Lett. 2016 Aug;38(8):1307-14. doi: 10.1007/s10529-016-2104-5. Epub 2016 Apr 12.
OBJECTIVES
To construct an Escherichia coli strain capable of producing riboflavin with high titer and yield.
RESULTS
A low copy number plasmid pLS01 containing a riboflavin operon under the control of a constitutive promoter was constructed and introduced into Escherichia coli MG1655. Subsequently, the pfkA, edd and ead genes were disrupted, and the resulting strain LS02T produced 667 mg riboflavin/l in MSY medium supplied with 10 g glucose/l in flask cultivation. In a fed-batch process, riboflavin production of the strain reached 10.4 g/l with a yield of 56.8 mg riboflavin/g glucose.
CONCLUSION
To our knowledge, this is the first report of engineered E. coli strains that can produce more than 10 g riboflavin/l in fed-batch cultivation, indicating that E. coli has potential for riboflavin production.
目的
构建能够高产核黄素的大肠杆菌菌株。
结果
构建了一个低拷贝质粒pLS01，其含有受组成型启动子控制的核黄素操纵子，并将其导入大肠杆菌MG1655。随后，破坏了pfkA、edd和ead基因，所得菌株LS02T在摇瓶培养中，于添加10 g/L葡萄糖的MSY培养基中产生667 mg/L核黄素。在补料分批培养过程中，该菌株的核黄素产量达到10.4 g/L，产率为56.8 mg核黄素/g葡萄糖。
结论
据我们所知，这是关于工程化大肠杆菌菌株在补料分批培养中能够产生超过10 g/L核黄素的首次报道，表明大肠杆菌在核黄素生产方面具有潜力。
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