蛋白质对核酸的选择性识别：鸟嘌呤与羧酸根离子相互作用的特异性
Selective recognition of nucleic acids by proteins: the specificity of guanine interaction with carboxylate ions.
作者信息
Lancelot G, Hélène C
出版信息
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4872-5. doi: 10.1073/pnas.74.11.4872.
Abstract
The interaction of carboxylate ions (acetate, butyrate) with nucleic aicd bases and nucleosides has been investigated by proton magnetic resonance in dimethyl sulfoxide (d6)/H2O mixtures. Carboxylate ions interacted only with guanine derivatives and led to a large downfield shift of the NH2 resonance. A 1:1 stoichiometry was deduced from a study of the concentration dependence of chemical shifts. A study of substituted guanine showed that hydrogen bonding involved N(1)H and NH2(2). An association constant of 110 M-1 was determined. This value is about 30 times higher than the association constant for guanine-cytosine base pair formation under the same experimental conditions. As a matter of fact, carboxylate ions induced a dissociation of guanine-cytosine base pairs. This guanine-carboxylate association is experimental evidence for a highly specific interaction that could play an important role in protein/nucleic acid recognition.
摘要
通过在二甲亚砜（d6）/H2O混合物中进行质子磁共振研究了羧酸根离子（乙酸根、丁酸根）与核酸碱基和核苷的相互作用。羧酸根离子仅与鸟嘌呤衍生物相互作用，并导致NH2共振发生大幅向低场位移。通过对化学位移浓度依赖性的研究推断出化学计量比为1:1。对取代鸟嘌呤的研究表明，氢键涉及N(1)H和NH2(2)。测定的缔合常数为110 M-1。该值比在相同实验条件下鸟嘌呤-胞嘧啶碱基对形成的缔合常数高约30倍。事实上，羧酸根离子诱导了鸟嘌呤-胞嘧啶碱基对的解离。这种鸟嘌呤-羧酸盐缔合是一种高度特异性相互作用的实验证据，该相互作用可能在蛋白质/核酸识别中起重要作用。
相似文献
1
Selective recognition of nucleic acids by proteins: the specificity of guanine interaction with carboxylate ions.蛋白质对核酸的选择性识别：鸟嘌呤与羧酸根离子相互作用的特异性
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4872-5. doi: 10.1073/pnas.74.11.4872.
2
Models of interaction between nucleic acids and proteins. Hydrogen bonding of arginine with nucleic acid bases, phosphate groups and carboxylic acids.核酸与蛋白质之间的相互作用模型。精氨酸与核酸碱基、磷酸基团和羧酸的氢键作用。
Biochim Biophys Acta. 1979 Sep 27;564(2):181-90. doi: 10.1016/0005-2787(79)90217-x.
3
Phosphate-guanosine interactions. A model for the involvement of guanine derivatives in autocatalytic reactions of ribonucleic acids.
J Biol Chem. 1984 Dec 25;259(24):15046-50.
4
5
Prominent stacking interaction with aromatic amino acid by N-quarternization of nucleic acid base: X-ray crystallographic characteristics and biological implications.通过核酸碱基的N-季铵化与芳香族氨基酸形成显著的堆积相互作用：X射线晶体学特征及生物学意义。
Arch Biochem Biophys. 1990 Apr;278(1):217-27. doi: 10.1016/0003-9861(90)90251-s.
6
Direct proton exchange between complementary nucleic acid bases.
Nature. 1978 Jan 19;271(5642):277-8. doi: 10.1038/271277a0.
7
STUDIES OF HYDROGEN-BONDED SYSTEMS. II. TUNNELING AND TAUTOMERIC EQUILIBRIA IN THE N-H...N HYDROGEN BOND OF THE GUANINE-CYTOSINE BASE PAIR.
J Chem Phys. 1965 Mar 15;42:2177-80. doi: 10.1063/1.1696263.
8
Nitrogen-15 chemical shifts in AT (adenine-thymine) and CG (cytosine-guanine) nucleic acid base pairs.腺嘌呤-胸腺嘧啶（AT）和胞嘧啶-鸟嘌呤（CG）核酸碱基对中的氮-15化学位移。
J Biomol Struct Dyn. 1998 Dec;16(3):619-29. doi: 10.1080/07391102.1998.10508274.
9
Proton magnetic resonance studies of specific association of nucleic acid constitutent bases in a nonaqueous solvent. Utility of DTBN radical to probe the affinity of hydrogen bonding involved in complementary base pairs1.
J Am Chem Soc. 1977 Jun 22;99(13):4299-4305. doi: 10.1021/ja00455a016.
10
[HALOGENATION OF NUCLEIC ACIDS. I. ACTION OF BROMINE ON BASES AND NUCLEOSIDES IN DIMETHYLFORMAMIDE MEDIUM].[核酸的卤化。I. 溴在二甲基甲酰胺介质中对碱基和核苷的作用]
Bull Soc Chim Biol (Paris). 1964;46:1059-71.
引用本文的文献
1
The importance of amino acid interactions in the crystallization of hydroxyapatite.氨基酸相互作用在羟基磷灰石结晶中的重要性。
J R Soc Interface. 2012 Dec 26;10(80):20120906. doi: 10.1098/rsif.2012.0906. Print 2013 Mar 6.
2
Sequence of events underlying the allosteric transition of rod cyclic nucleotide-gated channels.视杆细胞环核苷酸门控通道变构转变的潜在事件序列。
J Gen Physiol. 1999 May;113(5):621-40. doi: 10.1085/jgp.113.5.621.
3
A hypothesis: reciprocal information transfer between oligoribonucleotides and oligopeptides in prebiotic molecular evolution.一种假说：前生物分子进化中寡核糖核苷酸与寡肽之间的相互信息传递。
Orig Life Evol Biosph. 1994 Nov;24(6):495-505. doi: 10.1007/BF01582033.
4
Transfer-RNA, an early gene?转运核糖核酸，一种早期基因？
Naturwissenschaften. 1981 Jun;68(6):282-92. doi: 10.1007/BF01047470.
5
Molecular basis for the genetic code.遗传密码的分子基础。
J Mol Evol. 1982;18(5):297-303. doi: 10.1007/BF01733895.
6
Specific non-histone protein fractions associated with 'active' chromatin in immunized rats.与免疫大鼠中“活性”染色质相关的特定非组蛋白蛋白组分。
Mol Cell Biochem. 1982 Nov 26;49(2):119-28. doi: 10.1007/BF00242490.
7
Incorporation of various amino acids into non-histone chromatin protein fractions of spleen cells of mice immunized with IgG.用免疫球蛋白G免疫的小鼠脾细胞非组蛋白染色质蛋白组分中各种氨基酸的掺入情况。
Mol Cell Biochem. 1981 Jun 9;37(1):13-9. doi: 10.1007/BF02355883.
8
A selective recognition mode of a nucleic acid base by an aromatic amino acid: L-phenylalanine-7-methylguanosine 5'-monophosphate stacking interaction.芳香族氨基酸对核酸碱基的选择性识别模式：L-苯丙氨酸与7-甲基鸟苷5'-单磷酸的堆积相互作用。
Nucleic Acids Res. 1988 Jul 11;16(13):6175-90. doi: 10.1093/nar/16.13.6175.
9
Model studies of interactions between nucleic acids and proteins: hydrogen bonding of amides with nucleic acid bases.核酸与蛋白质相互作用的模型研究：酰胺与核酸碱基的氢键作用。
Nucleic Acids Res. 1979 Mar;6(3):1063-71. doi: 10.1093/nar/6.3.1063.
本文引用的文献
1
Hydrogen bonding of amino acid side chains to nucleic acid bases.氨基酸侧链与核酸碱基之间的氢键作用。
FEBS Lett. 1973 Mar 1;30(2):219-224. doi: 10.1016/0014-5793(73)80655-6.
2
The identification of a glutamic acid residue as part of the active site of ribonuclease T-1.将谷氨酸残基鉴定为核糖核酸酶T-1活性位点的一部分。
J Biol Chem. 1967 Oct 25;242(20):4682-90.
3
Proton magnetic resonance studies of self-association and metal complexation of nucleosides in dimethyl sulfoxide.
J Am Chem Soc. 1968 Sep 11;90(19):5069-74. doi: 10.1021/ja01021a003.
4
Nuclear magnetic resonance study of the interactions of guanosine and cytidine in dimethyl sulfoxide.二甲基亚砜中鸟苷与胞苷相互作用的核磁共振研究
J Am Chem Soc. 1968 Aug 28;90(18):5010-7. doi: 10.1021/ja01020a041.
5
Interactions of aromatic residues of proteins with nucleic acid. II. Proton magnetic resonance studies of the binding of tyramine and tyrosine-containing peptides to poly(adenylic acid) and deoxyribonucleic acid.蛋白质芳香族残基与核酸的相互作用。II. 酪胺及含酪氨酸肽与聚腺苷酸和脱氧核糖核酸结合的质子磁共振研究
Biochemistry. 1974 Feb 12;13(4):724-30. doi: 10.1021/bi00701a014.
6
Interactions of aromatic residues of proteins with nucleic acids. I. Proton magnetic resonance studies of the binding of tryptophan-containing peptides to poly(adenylic acid) and deoxyribonucleic acid.蛋白质芳香族残基与核酸的相互作用。I. 含色氨酸肽与聚腺苷酸和脱氧核糖核酸结合的质子磁共振研究。
Biochemistry. 1974 Feb 12;13(4):714-23. doi: 10.1021/bi00701a013.
7
Letter: Nucleoside complexing. A charge reversed chelate compound between guanosine and chloride ion.
J Am Chem Soc. 1974 May 29;96(11):3656-7. doi: 10.1021/ja00818a054.
8
Specific recognition of single-stranded regions in ultraviolet-irradiated and heat-denatured DNA by tryptophan-containing peptides.含色氨酸的肽对紫外线照射和热变性DNA中单链区域的特异性识别。
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3185-8. doi: 10.1073/pnas.71.8.3185.
9
[The possibility of recognition of nucleic bases by amino acids and peptides with the help of hydrogen bonds].
Mol Biol (Mosk). 1975 Mar-Apr;9(2):304-9.
10
Metal ion-mediated specific interactions between nucleic acid bases of polynucleotides and amino acid side chains of polypeptides.金属离子介导的多核苷酸核酸碱基与多肽氨基酸侧链之间的特异性相互作用。
Nucleic Acids Res. 1975 Jun;2(6):961-9. doi: 10.1093/nar/2.6.961.
Zotero 插件