Kiyama Masahiro, Saito Ryohei, Iwano Satoshi, Obata Rika, Niwa Haruki, Maki Shojiro A
Department of Engineering Science, The University of Electro-Communications, P.O. Box: 182-8585, Chofu, Tokyo, Japan.
Curr Top Med Chem. 2016;16(24):2648-55. doi: 10.2174/1568026616666160413135055.
Firefly bioluminescence is widely used in life science research as a useful analysis tool. For example, the adenosine-5`-triphosphate (ATP)-dependent enzymatic firefly bioluminescence reaction has long been utilized as a microbial monitoring tool. Rapid and sensitive firefly luciferin-luciferase combinations are used not only to measure cell viability but also for reporter-gene assays. Recently, bioluminescence was utilized as a noninvasive, real-time imaging tool for living subjects to monitor cells and biological events. However, the number of commercialized luciferase genes is limited and tissue-permeable near-infrared (NIR) region emitting light is required for in vivo imaging. In this review, recent studies describing synthetic luciferin analogues predicted to have red-shifted bioluminescence are summarized. Luciferase substrates emitting red, green, and blue light that were designed and developed in our laboratory are presented. The longest emission wavelength of the synthesized luciferin analogues was recorded at 675 nm, which is within the NIR region. This compound is now commercially available as "Aka Lumine®".
萤火虫生物发光作为一种有用的分析工具在生命科学研究中被广泛应用。例如，依赖三磷酸腺苷（ATP）的萤火虫生物发光酶促反应长期以来一直被用作微生物监测工具。快速且灵敏的萤火虫荧光素 - 荧光素酶组合不仅用于测量细胞活力，还用于报告基因检测。最近，生物发光被用作活体受试者的非侵入性实时成像工具，以监测细胞和生物事件。然而，商业化的荧光素酶基因数量有限，体内成像需要能穿透组织的近红外（NIR）区域发光。在这篇综述中，总结了描述预计具有红移生物发光的合成荧光素类似物的近期研究。展示了我们实验室设计和开发的发出红、绿、蓝光的荧光素酶底物。合成的荧光素类似物的最长发射波长记录在675nm，处于近红外区域。这种化合物现在作为“Aka Lumine®”商业可得。
