Zhang Jin-Sheng, Zhang Bao-Xia, Du Mei-Mei, Wang Xiao-Ya, Li Wei
First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
Third Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
Neural Regen Res. 2016 Feb;11(2):292-7. doi: 10.4103/1673-5374.177738.
After cerebral ischemia, bone marrow mesenchymal stem cells are mobilized and travel from the bone marrow through peripheral circulation to the focal point of ischemia to initiate tissue regeneration. However, the number of bone marrow mesenchymal stem cells mobilized into peripheral circulation is not enough to exert therapeutic effects, and the method by which blood circulation is promoted to remove blood stasis influences stem cell homing. The main ingredient of Xuesaitong capsules is Panax notoginseng saponins, and Xuesaitong is one of the main drugs used for promoting blood circulation and removing blood stasis. We established rat models of cerebral infarction by occlusion of the middle cerebral artery and then intragastrically administered Xuesaitong capsules (20, 40 and 60 mg/kg per day) for 28 successive days. Enzyme-linked immunosorbent assay showed that in rats with cerebral infarction, middle- and high-dose Xuesaitong significantly increased the level of stem cell factors and the number of CD117-positive cells in plasma and bone marrow and significantly decreased the number of CD54- and CD106-positive cells in plasma and bone marrow. The effect of low-dose Xuesaitong on these factors was not obvious. These findings demonstrate that middle- and high-dose Xuesaitong and hence Panax notoginseng saponins promote and increase the level and mobilization of bone marrow mesenchymal stem cells in peripheral blood.
脑缺血后，骨髓间充质干细胞被动员起来，从骨髓经外周循环迁移至缺血灶以启动组织再生。然而，动员进入外周循环的骨髓间充质干细胞数量不足以发挥治疗作用，且促进血液循环以活血化瘀的方法会影响干细胞归巢。血塞通胶囊的主要成分是三七总皂苷，血塞通是用于活血化瘀的主要药物之一。我们通过大脑中动脉闭塞建立大鼠脑梗死模型，然后连续28天每天给大鼠灌胃血塞通胶囊（20、40和60毫克/千克）。酶联免疫吸附测定显示，在脑梗死大鼠中，中、高剂量血塞通显著提高了血浆和骨髓中干细胞因子水平及CD117阳性细胞数量，并显著降低了血浆和骨髓中CD54和CD106阳性细胞数量。低剂量血塞通对这些因子的作用不明显。这些结果表明，中、高剂量血塞通以及三七总皂苷可促进并提高外周血中骨髓间充质干细胞的水平及动员能力。
